| Full text | |
| Author(s): Show less - |
Bolsoni-Lopes, Andressa
[1]
;
Festuccia, William T.
[1]
;
Farias, Talita S. M.
[1]
;
Chimin, Patricia
[1]
;
Torres-Leal, Francisco L.
[1]
;
Derogis, Priscilla B. M.
[2]
;
de Andrade, Paula B.
[1]
;
Miyamoto, Sayuri
[2]
;
Lima, Fabio B.
[1]
;
Curi, Rui
[1]
;
Alonso-Vale, Maria Isabel C.
[3]
Total Authors: 11
|
| Affiliation: | [1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo - Brazil
[3] Univ Fed Sao Paulo, Inst Environm Sci Chem & Pharmaceut, Dept Biol Sci, BR-09913030 Diadema - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM; v. 305, n. 9, p. E1093-E1102, NOV 2013. |
| Web of Science Citations: | 25 |
| Abstract | |
We investigated whether palmitoleic acid, a fatty acid that enhances whole body glucose disposal and suppresses hepatic steatosis, modulates triacylglycerol (TAG) metabolism in adipocytes. For this, both differentiated 3T3-L1 cells treated with either palmitoleic acid (16: 1n7, 200 mu M) or palmitic acid (16: 0, 200 mu M) for 24 h and primary adipocytes from wild-type or PPAR alpha-deficient mice treated with 16: 1n7 (300 mg.kg (-1.)day (-1)) or oleic acid (18: 1n9, 300 mg.kg (-1).day (-1)) by gavage for 10 days were evaluated for lipolysis, TAG, and glycerol 3-phosphate synthesis and gene and protein expression profile. Treatment of differentiated 3T3-L1 cells with 16:1n7, but not 16: 0, increased basal and isoproterenol-stimulated lipolysis, mRNA levels of adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) and protein content of ATGL and pSer(660)-HSL. Such increase in lipolysis induced by 16: 1n7, which can be prevented by pharmacological inhibition of PPAR alpha, was associated with higher rates of PPAR alpha binding to DNA. In contrast to lipolysis, both 16: 1n7 and 16: 0 increased fatty acid incorporation into TAG and glycerol 3-phosphate synthesis from glucose without affecting glyceroneogenesis and glycerokinase expression. Corroborating in vitro findings, treatment of wild-type but not PPAR alpha-deficient mice with 16: 1n7 increased primary adipocyte basal and stimulated lipolysis and ATGL and HSL mRNA levels. In contrast to lipolysis, however, 16: 1n7 treatment increased fatty acid incorporation into TAG and glycerol 3-phosphate synthesis from glucose in both wild-type and PPAR alpha-deficient mice. In conclusion, palmitoleic acid increases adipocyte lipolysis and lipases by a mechanism that requires a functional PPAR alpha. (AU) | |
| FAPESP's process: | 10/10909-8 - Role of adipose tissue in the development of obesity and associated co-morbidities: investigation of molecular mechanism and search for alternative therapies |
| Grantee: | William Tadeu Lara Festuccia |
| Support Opportunities: | Scholarships in Brazil - Young Researchers |
| FAPESP's process: | 11/51627-8 - Effect of fat acids on the differentiation and function of the adipose tissue |
| Grantee: | Maria Isabel Cardoso Alonso-Vale |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 09/15354-7 - Role of adipose tissue in the development of obesity and associated co-morbidities: investigation of molecular mechanism and search for alternative therapies |
| Grantee: | William Tadeu Lara Festuccia |
| Support Opportunities: | Research Grants - Young Investigators Grants |