Genotyping species of the Sporothrix schenckii complex by PCR-RFLP of calmodulin

Autor(es)

Rodrigues, Anderson Messias ^{[1, 2]} ; de Hoog, G. Sybren ^[1] ; de Camargo, Zoilo Pires ^[2]

Afiliação do(s) autor(es)

^[1] CBS KNAW Fungal Biodivers Ctr, Utrecht - Netherlands

^[2] Univ Fed Sao Paulo, Cellular Biol Div, Dept Microbiol Immunol & Parasitol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE; v. 78, n. 4, p. 383-387, APR 2014.
• Citações Web of Science: 31

Resumo

Sporotrichosis is one of the most common subcutaneous mycosis in Latin America and is caused by 4 pathogenic thermodimorphic fungi in the genus Sporothrix. From both therapeutic and epidemiological perspectives, it is essential to identify the causative agents down to the species level. Traditional parameters may overlap among closely related species, and we propose polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) as an alternative approach. In the present study, the calmodulin gene was amplified and digested with Hhal to yield 5 different electrophoretic patterns representing all medically important Sporothrix species: Sporothrix brasiliensis, Sporothrix schenckii sensu stricto, Sporothrix globosa, and Sporothrix luriei. The PCR-RFLP protocol described here is a simple and inexpensive method and is highly suitable for accurate routine genotyping of relevant Sporothrix species. (C) 2014 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 11/07350-1 - Biologia do complexo Sporothrix schenckii: Análise de marcadores moleculares e proteômicos e o papel de moléculas antigênicas e antifúngicas na esporotricose
• Beneficiário: Anderson Messias Rodrigues
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 09/54024-2 - Biologia molecular e proteômica de fungos de interesse médico: Paracoccidioides brasiliensis e Sporothrix schenckii
• Beneficiário: Zoilo Pires de Camargo
• Modalidade de apoio: Auxílio à Pesquisa - Temático