Stable SET knockdown in head and neck squamous cell carcinoma promotes cell invasion and the mesenchymal-like phenotype in vitro, as well as necrosis, cisplatin sensitivity and lymph node metastasis in xenograft tumor models

Autor(es)

Sobral, Lays M. ^[1] ; Sousa, Lucas O. ^{[2, 3]} ; Coletta, Ricardo D. ^[4] ; Cabral, Hamilton ^[5] ; Greene, Lewis J. ^{[2, 3]} ; Tajara, Eloiza H. ^[6] ; Gutkind, J. Silvio ^[7] ; Curti, Carlos ^[8] ; Leopoldino, Andreia M. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Riberiao Preto, Dept Clin Anal Toxicol & Food Sci, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Sch Med, Dept Cellular & Mol Biol, BR-14040903 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Sch Med Ribeirao Preto, INCT, Ctr Cell Therapy & Hemotherapy Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil

^[4] Univ Estadual Campinas, Piracicaba Dent Sch, Dept Oral Diag, Piracicaba, SP - Brazil

^[5] Univ Sao Paulo, Sch Pharmaceut Sci Riberiao Preto, Dept Pharmaceut Sci, BR-14040903 Ribeirao Preto, SP - Brazil

^[6] Sch Med Sao Jose do Rio Preto, Dept Mol Biol, Ribeirao Preto, SP - Brazil

^[7] Natl Inst Dent & Craniofacial Res, Oral & Pharyngeal Canc Branch, NIH, Bethesda, MD - USA

^[8] Univ Sao Paulo, Sch Pharmaceut Sci Riberiao Preto, Dept Chem & Phys, BR-14040903 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Molecular Cancer; v. 13, FEB 20 2014.
• Citações Web of Science: 26

Resumo

Background: SET/I2PP2A is a multifunctional protein that is up-regulated in head and neck squamous cell carcinoma (HNSCC). The action of SET in HNSCC tumorigenicity is unknown. Methods: Stable SET knockdown by shRNA (shSET) was established in three HNSCC cell lines: HN12, HN13, and Cal27. Protein expression and phosphorylated protein levels were determined by Western blotting and immunofluorescence, cell migration and invasion were measured by functional analysis, and PP2A activity was determined using a serine/threonine phosphatase assay. A real-time PCR array was used to quantify 84 genes associated with cell motility. Metalloproteinase (MMP) activity was assessed by zymographic and fluorometric assays. HN12shSET xenograft tumors (flank and tongue models) were established in Balb/c nude mice; the xenograft characteristics and cisplatin sensitivity were demonstrated by macroscopic, immunohistochemical, and histological analyses, as well as lymph node metastasis by histology. Results: The HN12shSET cells displayed reduced ERK1/2 and p53 phosphorylation compared with control. ShSET reduced HN12 cell proliferation and increased the sub-G1 population of HN12 and Cal27 cells. Increased PP2A activity was also associated with shSET. The PCR array indicated up-regulation of three mRNAs in HN12 cells: vimentin, matrix metalloproteinase-9 (MMP9) and non-muscle myosin heavy chain IIB. Reduced E-cadherin and pan-cytokeratin, as well as increased vimentin, were also demonstrated as the result of SET knockdown. These changes were accompanied by an increase in MMP-9 and MMP-2 activities, migration and invasion. The HN12shSET subcutaneous xenograft tumors presented a poorly differentiated phenotype, reduced cell proliferation, and cisplatin sensitivity. An orthotopic xenograft tumor model using the HN12shSET cells displayed increased metastatic potential. Conclusions: SET accumulation has important actions in HNSCC. As an oncogene, SET promotes cell proliferation, survival, and resistance to cell death by cisplatin in vivo. As a metastasis suppressor, SET regulates invasion, the epithelial mesenchymal transition, and metastasis. (AU)

• Processo FAPESP: 10/18544-9 - Análise do perfil de miRNAs associados a proteína SET e os seus efeitos na proliferação, invasão e migração em modelo de carcinoma espinocelular de boca usando modelos in vivo e in vitro
• Beneficiário: Lays Martin Sobral
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 09/52228-0 - Estudos da resistência à apoptose no câncer, modelo cabeça/pescoço: vias de sinalização com ênfase na PIP3-AKT/SET, estresse oxidativo, mitocôndria e correlações
• Beneficiário: Carlos Curti
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/08135-2 - CTC - Centro de Terapia Celular
• Beneficiário: Dimas Tadeu Covas
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 10/20384-0 - Identificação dos genes, miRNAs e proteínas regulados pela oncoproteína SET e envolvidos na malignização e progressão do carcinoma epidermóide de cabeça e pescoço usando modelo in vitro e in vivo
• Beneficiário: Andréia Machado Leopoldino
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/10898-4 - Estudo dos mecanismos moleculares associados à proteína SET com impacto na tumorigênese e progressão do câncer oral
• Beneficiário: Carlos Curti
• Modalidade de apoio: Auxílio à Pesquisa - Regular