8q Deletion in MYCN-amplified Neuroblastoma of a Child Born From Assisted Reproductive Technology

Autor(es)

Brassesco, Maria Sol ^[1] ; Valera, Elvis Terci ^[2] ; de Oliveira, Fabio Morato ^[3] ; de Paula Queiroz, Rosane Gomes ^[4] ; Scrideli, Carlos Alberto ^[5] ; Sakamoto-Hojo, Elza Tiemi ^[6] ; Tone, Luiz Gonzaga ^[7]

Afiliação do(s) autor(es)

^[1] USP. Fac Med Ribeirao Preto

^[2] USP. Fac Med Ribeirao Preto

^[3] USP. Fac Med Ribeirao Preto

^[4] USP. Fac Med Ribeirao Preto

^[5] USP. Fac Med Ribeirao Preto

^[6] USP. Fac Med Ribeirao Preto

^[7] USP. Fac Med Ribeirao Preto

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PEDIATRIC HEMATOLOGY / ONCOLOGY; v. 31, n. 3, p. 215-219, MAR 2009.
• Citações Web of Science: 3

Resumo

The occurrence of pediatric cancer in children born from assisted reproductive technologies has been sporadically reported. Chromosomal characterization of the neoplasic disease in this setting is poorly described. In the present study, neuroblastoma cells from a 13-month-old infant boy born after intracytoplasmatic sperm injection were characterized by combining conventional cytogenetics. fluorescence in situ hybridization (FISH), comparative genomic hybridization. and quantitative polymerase chain reaction methods. Cytogenetic analysis of neuroblastoma (NB) metaphase spreads at the time of diagnosis revealed numerous centromere-free extrachromosomal double minutes. suggesting high MYCN amplification. Comparative genomic hybridization analysis demonstrated the amplification of 2q24 similar to pter, with additional gain of the long arm of chromosome 17. Chromosome losses involved 8q, 9q, and 11q. No deletion of 1p was found. MYCN amplification was confirmed by quantitative polymerase chain reaction and fluorescence in situ hybridization analysis. This report describes several chromosomal abnormalities that were present in NB of a child born after intracytoplasmatic sperm injection. Besides some well described and prognostic genetic findings in NB as MYCN amplification, gain on 17q and losses oil 9q and 11q23, we report an unusual deletion involving 8q region in this disease. Whether this genetic abnormality may be associated to assisted reproductive technologies deserves further investigation. (AU)

• Processo FAPESP: 06/04827-3 - Análise de aneuploidias por hibridação genômica comparativa (CGH) em pacientes pediátricos portadores de leucemia linfóide aguda
• Beneficiário: María Sol Brassesco Annichini
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 07/54236-4 - Analise do perfil genomico em neoplasias da infancia atraves da hibridizacao genomica comparativa.
• Beneficiário: Luiz Gonzaga Tone
• Modalidade de apoio: Auxílio à Pesquisa - Regular