Effects of using sodium hypochlorite mixtures with different chelating agents associated with or without passive ultrasonic agitation on dentin cleaning and extrusion of debris, on cell viability, tissue inflammation and demetalization of biofilms.

• Grant number: 17/22364-5
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2018
• End date: December 31, 2021
• Field of knowledge: Health Sciences - Dentistry - Endodontics
• Principal Investigator: Marco Antonio Hungaro Duarte
• Grantee: Marco Antonio Hungaro Duarte
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Associated researchers: Luciano Tavares Angelo Cintra ; Rodrigo Cardoso de Oliveira

Abstract

The chelating agents Etidronate (HEDP) and Tetrasodium EDTA alkali (EDTANa4) recently were suggested as substitutes for EDTA in removing smear layer, therefore they possess the advantage they can be used mixed with sodium hypochlorite (NaOCl) in the biomechanical preparation of root canal system without compromising the dissolution of organic matter and the antimicrobial action of NaOCl. These substances may exert antimicrobial effect, if they are capable of chelating metals essential for microbial metabolism and stability of biofilm. The EDTANa4 is only available for use in research, but at the beginning of this year commercial form of HEDP is available in Europe, studies that evaluate biological effects of these rinsing become extremely relevant. This project will be useful to analyze the effects of mixtures of HEBP and EDTANa4 with NaOCl associated with ultrasonic agitation passive (PUI) on different aspects of the Endodontic treatment and to verify the effects of such as the contact with peripicais and rinsing in the demetalization of biofilms. Therefore, this project aims to: 1. Evaluate the effect adas HEBP blends and EDTANa4 with NaOCl associated with ultrasonic agitation passive (PUI) at debris removal of root canals and in extrusion of debris during instrumentation; 2. Evaluate the effect of HEDP, EDTANa4 and other rinsing on cell viability in NIH-3T3 cells culture and MC3T3-E1, in the capacity of mineralization, in the expression of pro-inflammatory cytokine genes by RT-PCR and the production of Pro-Inflammatory Cytokines by Western-blot MC3T3-E1 cells; 3. Evaluate the inflammatory response in rat subcutaneous by hematoxylin-eosin and Immunohistochemistry; 4. Develop a methodology to assess the order of biofilms by these chelating agents in confocal laser scanning microscopy. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

TARTARI, T.; BORGES, M. M. B.; DE ARAUJO, L. B. B.; VIVAN, R. R.; BONJARDIM, L. R.; DUARTE, M. A. H.. Effects of heat in the properties of NaOCl alone and mixed with etidronate and alkaline tetrasodium EDTA. International Endodontic Journal, v. 54, n. 4, p. 616-627, APR 2021. (18/12690-5, 17/22364-5)

TALITA TARTARI; CAROLINE WICHNIESKI; RENATO MENEZES SILVA; ARIADNE LETRA; MARCO ANTONIO HUNGARO DUARTE; CLOVIS MONTEIRO BRAMANTE. Final irrigation protocols can be used to promote stable long-term bond strength of AH Plus to dentin. Journal of Applied Oral Science, v. 31, 2023-05-26. (15/09958-8, 18/12690-5, 13/19789-3, 17/22364-5)