In vitro evaluation of lipogenics and anti-adipogenics substances using three-dimensional model of adipocytes

• Grant number: 17/26618-1
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2018
• End date: May 31, 2021
• Field of knowledge: Health Sciences - Pharmacy - Medicines Analysis and Control
• Principal Investigator: Patricia Santos Lopes
• Grantee: Patricia Santos Lopes
• Host Institution: Instituto de Ciências Ambientais, Químicas e Farmacêuticas (ICAQF). Universidade Federal de São Paulo (UNIFESP). Campus Diadema. Diadema , SP, Brazil

Abstract

The hyperplasia and hypertrophy of the adipocytes, generates a lot of dissatisfaction, especially in women, so research that reorganize the adipose tissue are frequent and necessary. Currently there is no in vitro model to test formulations for the treatment of cellulite and adipocyte dysfunctions, described in literature, even with the implementation of international and national laws and decrees urging the discontinuance of the use of animals in tests focused on ingredients and/or cosmetic products. Derived from plants and synthetic substances are widely used in cosmetic formulations due to multifunctional features that elevate the action potential. However, for the public use a screening must be conducted with regard to safety and efficacy. The goals of this proposal include the safety evaluation tests, based on the tests recommended by the OECD, such as cytotoxicity (OECD/GD 129), Phototoxicity (OECD 432) and the micronucleus in vitro (OECD 432). The efficacy test will be performed in three-dimensional levitation cell culture system based on magnetic particles which is the model ex vivo that it seems closer to the morphology and tissue interactions whereby in vivo. Thus, it will be possible to come up with an in vitro model to provide proof of effect claimed in cosmetics actives with the aim of replacement animal uses in research and development of cosmetic products. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

LOSITO, DANILO W.; LOPES, PATRICIA S.; UEOKA, ANDREZA R.; FANTINI, MARCIA C. A.; OSELIERO FILHO, PEDRO LEONIDAS; ANDREO-FILHO, NEWTON; MARTINS, TEREZA S.. Biocomposites based on SBA-15 and papain: Characterization, enzymatic activity and cytotoxicity evaluation. Microporous and Mesoporous Materials, v. 325, OCT 2021. (19/12301-1, 17/26618-1, 17/17844-8, 16/22916-5, 20/13204-7, 19/08582-5)