Mycobioma of soil samples from central-west region of São Paulo by Next-Generation Sequencing(NGS): Analysis of pathogenic species distribution

• Grant number: 18/06390-9
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2018
• End date: October 31, 2020
• Field of knowledge: Biological Sciences - Microbiology - Applied Microbiology
• Principal Investigator: Eduardo Bagagli
• Grantee: Eduardo Bagagli
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Despite the recognized importance of fungi (Eumycota) in biotechnological processes and clinical area, the ecology of these organism remains poorly understood, mainly due to difficulties for isolating these fungi from the environment by culture techniques. In this context, Next-Generation Sequencing (NGS) has emerged revolutionizing Microbiology. This technique consists of a high coverage sequencing of the total environmental DNA (metagenome) that allows a broad view on the structure, diversity and function of the microbial community, with no need of fungal isolation. The technique has been widely used for a more robust understanding of the ecology of microorganisms and mycobiota studies. Ecology is essential for understanding many diseases caused by pathogenic fungi, especially species of the order Onygenales, endemic fungi, which have a more restricted distribution and favored growth in environments with abundant organic matter (keratin) and protected of UV radiation, temperature variation and rough weather conditions. This order encompasses fungi of the genus Paracoccidioides, Histoplasma and Coccidioides, responsible for important systemic mycoses in Latin America. In this work, we will evaluate the fungal diversity in soil samples from the central-west region of São Paulo, by NGS, establishing comparisons among the different environments. We hope to gain a better understanding of the determinant factors related to the environmental distribution of mycobiota, especially in groups of clinical interest, contributing for adopting possible prevention measures. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BAGAGLI, EDUARDO; MATUTE, DANIEL RICARDO; GARCES, HANS GARCIA; TENORIO, BERNARDO GUERRA; GARCES, ADALBERTO GARCIA; ALVES, LUCAS GOMES DE BRITO; YAMAUCHI, DANIELLE HAMAE; HRYCYK, MARLUCE FRANCISCA; BARKER, BRIDGET MARIE; TEIXEIRA, MARCUS DE MELO. Paracoccidioides brasiliensis Isolated from Nine-Banded Armadillos (Dasypus novemcinctus) Reveal Population Structure and Admixture in the Amazon Basin. JOURNAL OF FUNGI, v. 7, n. 1, JAN 2021. (18/06390-9)

YAMAUCHI, DANIELLE HAMAE; GARCES, HANS GARCIA; TEIXEIRA, MARCUS DE MELO; BARROS RODRIGUES, GABRIEL FELLIPE; ULLMANN, LEILA SABRINA; GARCES, ADALBERTO GARCIA; HEBELER-BARBOSA, FLAVIA; BAGAGLI, EDUARDO. Soil Mycobiome Is Shaped by Vegetation and Microhabitats: A Regional-Scale Study in Southeastern Brazil. JOURNAL OF FUNGI, v. 7, n. 8, AUG 2021. (18/06390-9)