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Ecotoxicological evaluation of water from the Sorocaba river using an integrated analysis of biochemical and morphological biomarkers in bullfrog tadpoles, Lithobates catesbeianus (Shaw, 1802)

Grant number: 17/23781-9
Support type:Regular Research Grants
Duration: September 01, 2018 - August 31, 2020
Field of knowledge:Biological Sciences - Biochemistry
Principal Investigator:Cleoni dos Santos Carvalho
Grantee:Cleoni dos Santos Carvalho
Home Institution: Centro de Ciências Humanas e Biológicas (CCHB). Universidade Federal de São Carlos (UFSCAR). Sorocaba , SP, Brazil
Assoc. researchers:Lilian Franco Belussi ; Marisa Narciso Fernandes

Abstract

The aquatic environment is continuously subject to toxic contaminants as a result of industrial and domestic activities. The evaluation of the effects these contaminants on aquatic organisms at different levels, that are, cellular, tissue, biochemical and molecular, will contribute to the predictability of the effects caused by different pollutants in the environment, generating information about mechanisms of adaptation to environmental stressors. Thus, this work has the objective of investigating the organs (liver, kidney, muscle, brain and skin) of bullfrog tadpoles, Lithobates catesbeianus, if the exposure to water of the Sorocaba River can impose limits in the performance of the enzymatic and metabolic activities impairing thus skills such as growth and reproduction. The experimental project will consist of submitting groups of animals in the water of the Sorocaba River in the dry and rainy periods at five days. The presence of metals, agrochemicals and drugs will be determined in samples of waters of the Sorocaba River in the two periods and in two points. For an evaluation of the effects, the parameters will be analyzed: genotoxic analyzes and nuclear abnormalities in the peripheral blood of the animals; activity of antioxidant enzymes: SOD (superoxide dismutase), CAT (catalase), GPx (glutathione peroxidase), GR (glutathione reductase) and GST (glutathione S-transferase); levels of PCO (carbonyl proteins) and MT (metallothionein); activity of NOS (nitric oxide synthase) and AChE (acetylcholinesterase) (brain and muscle); concentration of MXR (multixenobiotic resistance system) proteins (liver and kidney) and determination of the activity of enzymes involved in the glucose metabolism: HK (hexokinase), PFK (phosphofructokinase), PK (pyruvate kinase) and LDH (lactate dehydrogenase). The levels of glucose, glycogen, lipid and protein are also determined in the different organs. Morphological biomarkers and hepatic catabolic substances (hemosiderine and lipofuscine) will also be investigated and may be correlated with lipid metabolism and erythrocyte degradation. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CARVALHO, CLEONI DOS SANTOS; FERNANDES, MARISA NARCISO. Effects of copper toxicity at different pH and temperatures on the in vitro enzyme activity in blood and liver of fish, Prochilodus lineatus. MOLECULAR BIOLOGY REPORTS, v. 46, n. 5, p. 4933-4942, OCT 2019. Web of Science Citations: 0.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.