High density genome wide genotyping on a pedigree of AIRmin and AIRmax mice to map and characterize new loci involved in lung and intestinal tumour development

• Grant number: 17/50407-0
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2018
• End date: August 31, 2019
• Field of knowledge: Biological Sciences - Immunology - Immunogenetics
• Mobility Program: SPRINT - Projetos de pesquisa - Mobilidade
• Principal Investigator: Olga Celia Martinez Ibanez
• Grantee: Olga Celia Martinez Ibanez
• Principal researcher abroad: Tommaso Antonio Dragani
• Institution abroad: Istituto Nazionale per lo Studio e la Cura dei Tumori, Italy
• Host Institution: Instituto Butantan. Secretaria da Saúde (São Paulo - Estado). São Paulo , SP, Brazil
• Associated research grant: 16/07007-9 - Analysis of the phenotypic effect of SNPs mapping at Irm1 locus which controls IL-1beta production in the mouse., AP.R

Abstract

Two non-inbred mouse lines, phenotypically selected for resistance (AIRmin) and susceptibility (AIRmax) to acute inflammatory response, show differential susceptibility/resistance to the development of several tumour types after chemical carcinogen treatment. An intercross pedigree of these mice has been generated and treated with chemical carcinogen dimethyl hydrazine, able to induce lung and intestinal tumours, at Instituto Butantan, Brazil. Thanks to collaboration with the Istituto Nazionale dei Tumori, Italy, the present project aims to carry out genome wide high-density genotyping with the innovative 2B-RAD technique to map genetic loci modulating multiple, phenotypes: individual genetic susceptibility to lung and intestinal cancer, and to acute inflammation. Moreover, it is planned to carry out a transcriptome analysis on lung tumour tissue and to map the expression quantitative traits loci (eQTL) that modulate the expression of genes related to the lung tumour phenotype. Overall, results of the project will provide an improved understanding of the relationship between genomic variation, individual genetic predisposition to tumourigenesis, and control of gene expression in tumours. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BORREGO, ANDREA; JENSEN, JOSE RICARDO; KOURY CABRERA, WAFA HANNA; MASSA, SOLANGE; RIBEIRO, ORLANDO GARCIA; STAROBINAS, NANCY; DE FRANCO, MARCELO; ETO, SILAS FERNANDES; MANENTI, GIACOMO; DRAGANI, TOMMASO ANTONIO; et al. Mapping of novel loci involved in lung and colon tumor susceptibility by the use of genetically selected mouse strains. Genes and Immunity, DEC 2021. (17/50407-0)