Effect of low level laser therapy on oxidative stress and DNA-damage response in oral mucosa cells exposed to chemotherapy agents and gamma radiation: in vitro study

• Grant number: 18/19028-6
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2019
• End date: January 31, 2022
• Field of knowledge: Health Sciences - Dentistry
• Principal Investigator: Luciana Corrêa
• Grantee: Luciana Corrêa
• Host Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Maria Cristina Zindel Deboni ; Maria Fátima Guarizo Klingbeil ; Monica Beatriz Mathor

Abstract

Low level laser therapy has been adopted for the prevention and treatment of oral mucositis. This lesion is derived from an intense oxidative stress caused by radiotherapy and chemotherapy. The oxidative stress induces the formation of oxidized bases and activates a DNA-damage response (DDR) in neoplastic and normal cells. The DDR is composed mainly by mechanisms of non-homologous end-joining, homologous recombination, and base excision. The effect of low level laser therapy on oxidative stress and DDR in oral keratinocytes exposed to chemotherapy and radiotherapy was not addressed yet. In this study we aim to verify whether the laser therapy with different energy parameters (0,2J - 6,6J/cm2; 0,4J - 13,3J/cm2; 1J - 33,3J/cm2, 2J - 66,6J/cm2) reduces the oxygen reactive species and oxidized bases, as well as alters the expression of antioxidative proteins in normal oral keratinocytes treated with gamma radiation or chemotherapy agents (5- fluorouracil, melphalan, and methotrexate). We aim also to investigate whether the laser therapy reduces the DNA fragmentation and modifies the expression of DDR-related proteins (g-H2AX, ERCC1, OGG1, ATM e p53). Our intention is to demonstrate whether laser therapy can reduce the genotoxic effect of antineoplastic agents in normal oral keratinocytes and what energy range is more appropriate. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PERILLO ROSIN, FLAVIA CRISTINA; GONSALVES, HELOISA; SANTOS, ANAELIZA FIGUEIREDO; NOVAES, CRISTINA DE PAULA; HUANG, ISAAC; ZINDEL DEBONI, MARIA CRISTINA; CORREA, LUCIANA. Keratin expression in gingival tissue and primary cultured gingival keratinocytes: Are there differences?. ARCHIVES OF ORAL BIOLOGY, v. 117, SEP 2020. (18/19028-6)

PERILLO ROSIN, FLAVIA CRISTINA; NOVAES, CRISTINA DE PAULA; DOS SANTOS, ANAELIZA FIGUEIREDO; ZINDEL DEBONI, MARIA CRISTINA; CORREA, LUCIANA. Photobiomodulation Therapy Minimizes the DNA Damage in 5FU-Treated Gingival Fibroblasts. Photochemistry and Photobiology, v. 98, n. 5, p. 6-pg., 2022-03-05. (18/19028-6)