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Expression of cellular death markers in premalignant lesions induced in rat tongue and treated with photodynamic therapy

Grant number: 11/07437-0
Support type:Regular Research Grants
Duration: October 01, 2011 - October 31, 2013
Field of knowledge:Health Sciences - Dentistry
Principal Investigator:Luciana Corrêa
Grantee:Luciana Corrêa
Home Institution: Faculdade de Odontologia (FO). Universidade de São Paulo (USP). São Paulo , SP, Brazil


The use of photodynamic therapy (PDT) in treatment of oral premalignant lesions (OPM) has been focused on recent researches. PDT is based on an electromagnetic interaction with a photo sensitizer and tissue oxygen, forming reactive oxygen species (ROS) that cause death in metabolically active cells. The aim of this study is to establish the anatomic and chronologic relationship of cellular death in PDT mediated by aminolevulinic acid (ALA) performed in rat chemical-induced OPM. Topical application of 4-nitroquinoline-1-oxide (4NQO) will be made weekly in the tongue mucosa of rats until the appearance of white lesions. These lesions will be treated topically with ALA and then irradiate with low power laser (660nm, 1.5 min, 40mW, 3.6J, 90J/cm2). The animals will be euthanized at 6h, 24h, 48h and 5 days after the TFD. Immunohistochemical and Western blot analysis of beclin-1 protein (for analysis of autophagia), caspase 3, NF-kB p65 (for analysis of apoptosis and anti-apoptosis), RIP-1 (for analysis of necrosis), superoxide dismutase-1 (SOD-1), and inducible oxide nitrite sintase (iNOS) (for analysis of ROS) will be analyzed. Anti CD-34 positive blood vessels will be counted for analysis of vascular network. It is expected that the TFD improves the expression of death and oxidative stress proteins and reduces the vascular network as a time-dependant mechanism of cell proliferation control. By these results, it will be possible to better understand the death mechanisms in relation to the time, which may permit the development of TFD protocols using the topical application for OPM. (AU)

Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
TEIXEIRA BUCK, MARINA GABRIELA; CABRAL TUCI, PRISCILA SOUZA; PERILLO ROSIN, FLAVIA CRISTINA; PINHEIRO BARCESSAT, ANA RITA; CORREA, LUCIANA. Immunohistochemistry profile of p75 neurotrophin receptor in oral epithelial dysplasia and oral squamous cell carcinoma induced by 4-nitroquinoline 1-oxide in rats. ARCHIVES OF ORAL BIOLOGY, v. 96, p. 169-177, DEC 2018. Web of Science Citations: 0.
PERILLO ROSIN, FLAVIA CRISTINA; BARCESSAT, ANA RITA RIBEIRO; GIOVANNI BORGES, GIULIANA GADONI; VALENTE FERREIRA, LUCIANA GONCALVES; CORREA, LUCIANA. Vascular alterations after photodynamic therapy mediated by 5-aminolevulinic acid in oral leukoplakia. Lasers in Medical Science, v. 32, n. 2, p. 379-387, FEB 2017. Web of Science Citations: 2.
PERILLO ROSIN, FLAVIA CRISTINA; PINHEIRO BARCESSAT, ANA RITA; BORGES, GIULIANA GADONI; CORREA, LUCIANA. Effect of 5-ALA-mediated photodynamic therapy on mast cell and microvessels densities present in oral premalignant lesions induced in rats. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 153, p. 429-434, DEC 2015. Web of Science Citations: 1.
RIBEIRO BARCESSAT, ANA RITA; HUANG, ISAAC; RABELO, GUSTAVO DAVI; PERILLO ROSIN, FLAVIA CRISTINA; VALENTE FERREIRA, LUCIANA GONCALVES; DE CERQUEIRA LUZ, JOAO GUALBERTO; CORREA, LUCIANA. Systemic toxic effects during early phases of topical 4-NQO-induced oral carcinogenesis in rats. JOURNAL OF ORAL PATHOLOGY & MEDICINE, v. 43, n. 10, p. 770-777, NOV 2014. Web of Science Citations: 1.
BARCESSAT, ANA RITA; HUANG, ISAAC; ROSIN, FLAVIA PERILLO; PINTO, JR., DECIO DOS SANTOS; ZEZELL, DENISE MARIA; CORREA, LUCIANA. Effect of topical 5-ALA mediated photodynamic therapy on proliferation index of keratinocytes in 4-NQO-induced potentially malignant oral lesions. JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY, v. 126, p. 33-41, SEP 5 2013. Web of Science Citations: 7.

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