Supplementation with two Amazonian plants with proven immunostimulating effect in Nile tilapia

• Grant number: 18/06137-1
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2019
• End date: March 31, 2021
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Principal Investigator: Julieta Rodini Engracia de Moraes
• Grantee: Julieta Rodini Engracia de Moraes
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated researchers: Diogo Teruo Hashimoto ; Jefferson Yunis Aguinaga

Abstract

Minthostachys mollis and Myrciaria dubia are medicinal plants of known immunostimulating action. Previous studies conducted in our research group have demonstrated that dietary supplementation with these plants improves the immune response and growth of fish. However, there are variables such as time of supplementation, fish age, and identification of the active compounds that were not previously studied. The aim of this project is to continue the research carried out to improve the use of the extract of these plants in Nile tilapia food supplementation. For this, 960 Nile tilapia, Oreochromis niloticus, with an average weight of 150g will be used in both tests. In the first one (M. mollis), Nile tilapia will be distributed randomly in four experimental groups (10 fish per box in triplicate). The fish will be fed for a period of 30 days with three different experimental diets containing 0.5%, 1.0% and 2.0% of essential oil of this plant in the ration, in addition to a basal diet without the supplementation used as control. In the second trial (M. dubia), it will be evaluated if the effects observed by the supplementation of this plant for 5 weeks are due to vitamin C content or if there is participation of the other active compounds. For this, naive control group (not supplemented with camu-camu) supplemented with 100 and 500 mg / kg of camu-camu and supplemented with the equivalent value of vitamin C content of the groups of 100 and 500 mg / kg of camu-camu. In both cases, after the completion of supplementation the animals will be inoculated into the swim bladder with the Streptococcus agalactiae LD50 and evaluated at 6, 24 and 48 hours post infection. The tests will be performed in triplicate in a completely randomized design. After the aforementioned times, samples will be taken to evaluate the hematology, aerocystitis effect, serum biochemistry, cortisolemia, lipid peroxidation, blood glucose, leukocyte respiratory activity, lysozyme concentration, serum lytic activity, bacterial agglutination activity, complement system: hemolytic activity, histopathology and immunohistochemistry. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

RAMOS-ESPINOZA, FERNANDO CARLOS; CUEVA-QUIROZ, VICTOR ALEXANDER; YUNIS-AGUINAGA, JEFFERSON; ALVAREZ-RUBIO, NORQUIS CALED; DE MELLO, NICOLI PAGANOTI; ENGRACIA DE MORAES, JULIETA RODINI. Efficacy of two adjuvants administrated with a novel hydrogen peroxide-inactivated vaccine against Streptococcus agalactiae in Nile tilapia fingerlings. FISH & SHELLFISH IMMUNOLOGY, v. 105, p. 9-pg., 2020-10-01. (18/06137-1, 19/02339-1)

ALVAREZ-RUBIO, NORQUIS CALED; YUNIS-AGUINAGA, JEFFERSON; CALA-DELGADO, DANIEL L.; CUEVA QUIROZ, VICTOR ALEXANDER; MIANI VERRI, BRUNO LUIS; RAMOS ESPINOZA, FERNANDO CARLOS; ENGRACIA DE MORAES, JULIETA RODINI. Influence of hand net mesh type and sex on experimental infection with Aeromonas hydrophila in Brazilian native fish Astyanax altiparanae. AQUACULTURE REPORTS, v. 16, MAR 2020. (18/06137-1, 16/18345-2)

RAMOS-ESPINOZA, FERNANDO CARLOS; CUEVA-QUIROZ, VICTOR ALEXANDER; YUNIS-AGUINAGA, JEFFERSON; ENGRACIA DE MORAES, JULIETA RODINI. A comparison of novel inactivation methods for production of a vaccine against Streptococcus agalactiae in Nile tilapia Oreochromis niloticus. Aquaculture, v. 528, NOV 15 2020. (18/06137-1, 16/18345-2, 19/02339-1)