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Dietary supplementation of glutamine on growth performance, intestinal morphology, hematic, enzymatic and immunological responses of the Nile Tilapia subjected to bacterial challenge

Grant number: 14/01973-5
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): July 01, 2014
Effective date (End): February 02, 2017
Field of knowledge:Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
Principal researcher:Margarida Maria Barros
Grantee:Pedro Luiz Pucci Figueiredo de Carvalho
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Associated scholarship(s):15/23777-6 - Dietary supplementation of glutamine on growth performance, intestinal morphology, hematic, enzymatic and immunological responses of the Nile tilapia subjected to bacterial challenge, BE.EP.DR


Two experiments will be conducted to evaluate the effect of glutamine supplementation in Nile tilapia diets. Study I: aims to determine the optimal level of glutamine supplementation through productive performance responses, body composition, haematological, immunological, biochemical responses and intestinal morphology. Four hundred masculinized Nile tilapia juveniles (5g) will be distributed in 40 tanks (250 L each), in a recirculating water system. The experimental design will be completely randomized with five treatments (0, 0.5, 1.0, 1.5 and 2.0 % glutamine ) and eight replicates. The fish will be hand fed four times a day for 90 days. Study II: the contribution of glutamine supplementation in haematological, immunological and biochemical responses will be assessed after bacterial challenge by Aeromonas hydrophila. After the productive performance test, 60 fish (150 g) will be inoculated intraperitoneally with pathogen and transferred to experimental challenge room containing 30 tanks of 40 liters each, making six replications per treatment in a completely randomized design. At the end of 15 days, haematological, immunological and biochemical parameters of these animals will be analyzed. The data will be submitted to analysis of variance. In case of differences (P < 0.05), will be subjected to regression analysis and compared using the Tukey test. Variables that did not follow normal distribution will be submitted to the Kruskal - Wallis test, complemented by the Mann-Whitney test. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CARVALHO, PEDRO L. P. F.; YAMAMOTO, FERNANDO Y.; BARROS, MARGARIDA M.; GATLIN, III, DELBERT M. L-glutamine in vitro supplementation enhances Nile tilapia Oreochromis niloticus (Linnaeus, 1758) leukocyte function. FISH & SHELLFISH IMMUNOLOGY, v. 80, p. 592-599, SEP 2018. Web of Science Citations: 1.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
CARVALHO, Pedro Luiz Pucci Figueiredo de. Suplementação dietética de glutamina no desempenho produtivo, morfologia intestinal, respostas hemáticas, enzimáticas e imunológicas da tilápia-do-Nilo submetida a desafio bacteriano. 2017. Doctoral Thesis - Universidade Estadual Paulista "Júlio de Mesquita Filho" Universidade Estadual Paulista (UNESP), Faculdade de Medicina Veterinária e Zootecnia, Botucatu.

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