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Marine colagen from sponges: evaluation of genotoxicity and biocompatibility for bone tissue engineering

Grant number: 18/03759-1
Support type:Regular Research Grants
Duration: May 01, 2019 - April 30, 2020
Field of knowledge:Interdisciplinary Subjects
Principal Investigator:Ana Claudia Muniz Renno
Grantee:Ana Claudia Muniz Renno
Home Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil
Assoc. researchers:Ingrid Regina Avanzi ; Julia Risso Parisi ; Kelly Rossetti Fernandes ; Renata Neves Granito

Abstract

The healing of large dimension fractures due to traumas, malignancy or congenital diseases are a great challenge to the medicine field. Biomaterials have been arising as an alternative to be used as a graft to bone repair, which can stimulate bone metabolism and accelerate tissue regeneration, decreasing costs and reducing morbidity and mortality. In this context, the osteogenic and bioactive properties of synthetic materials such as hydroxyapatite and Biosilicate glass can be highlighted. In addition, bone grafts made from natural materials, especially from marine specimens, have also been widely used. Thus, the aim of this study is to develop and to verify the biological performance of a marine collagen. For this purpose, this study will characterize, evaluate the biocompatibility and biological effects of marine collagen from the sponge Aplysina caissara. The scanning electron microscope analysis (SEM) and Fourier transform infrared spectroscopy (FTIR) will be used to verify the physical properties and structure of the materials. For the in vitro tests, the cytotoxicity and genotoxicity of the composites will be evaluated by Alamar Blue and Comet assay. For the in vivo tests, Wistar rats will have samples implanted in a cranial bone deffect, which will be filled with the biomaterials. The animals will be divided into groups: control and marine collagen. Each experimental group will be euthanized after 3, 7, 15 and 45 days post-surgery. To verify the effects of the biomaterials, the follow analysis will be performed: histopathology, morphometry, immunohistochemistry analysis. It is expected that the present study will contribute to the development of new biomaterials to be used as bone grafts, resulting in new approaches to the treatment of bone fractures. (AU)