The role of sugar-responsive bZIP transcription factors in the regulation of the circadian oscillator of Arabidopsis

• Grant number: 18/25710-4
• Support Opportunities: Research Projects - Thematic Grants
• Start date: February 01, 2019
• End date: January 31, 2026
• Field of knowledge: Biological Sciences - Genetics - Plant Genetics
• Agreement: BBSRC, UKRI
• Principal Investigator: Michel Georges Albert Vincentz
• Grantee: Michel Georges Albert Vincentz
• Principal researcher abroad: Alexander Arundell Ross Webb
• Institution abroad: University of Cambridge, England
• Host Institution: Centro de Biologia Molecular e Engenharia Genética (CBMEG). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Américo José Carvalho Viana ; Carlos Takeshi Hotta
• Associated scholarship(s): 24/05144-5 - Investigation of the interaction between bZIP, bZIP1, bZIP53 and bZIP63 transcription factors, BP.TT; 24/05876-6 - The regulatory effects of the transcription factor AtbZIP63 on the expression of target genes in complemented Arabidopsis thaliana lines., BP.IC; 23/11182-4 - Defining the role of transcriptional regulation factors bZIP1, bZIP53, and bZIP63 in maintaining energy balance, BP.PD; + associated scholarships 22/03300-4 - The role of sugar-responsive bZIP transcription factors in the regulation of the circadian oscillator of Arabidopsis, BP.TT; 21/05512-6 - The transcription factor AtbZIP63: importance of regulating its abundance in the management of energy homeostasis in Arabidopsis thaliana, BP.DD; 20/10077-4 - The role of sugar-responsive bZIP transcription factors in the regulation of the circadian oscillator of Arabidopsis, BP.TT; 19/25696-4 - Define the role of bZIP1, bZIP53 and bZIP63 transcriptional regulatory factors in maintaining energy balance, BP.PD; 20/00304-3 - Defining the role of the heterodimerization network involving the transcription regulatory factors bZIP1, bZIP53 and bZIP63 in the control of energy homeostasis focusing on starch degradation, BP.DD - associated scholarships

Abstract

Biological circadian clock is a fundamental device which synchronize the rhythmic physiological functions and developmental decisions with the environmental day-night cycle and temperature fluctuations. Optimization of Plant biology rhythms is important for productivity and requires that the circadian clock is correctly synchronized with daily environmental changes. This process, which is known as entrainment, occurs every day through circadian phase adjustment in response to cues including light, temperature and sugars produced by photosynthesis. The mechanisms underlying entrainment are not well understood. The main objective of this project is to investigate into more details the mechanisms by which sugars/energy entrain the clock. We have shown in Arabidopsi thaliana that energy- and sugar-signalling network involve the bZIP type transcription factor bZIP63. This factor directly regulates the clock gene PRR7 to adjust the period and phase of the circadian oscillator in response to sugars in Arabidopsis. We now wish to define the regulatory network by which bZIP63 and its dimerization partners bZIP1 ad bZIP53 regulate the clock to manage energy homeostasis.This will be achieved by meeting the following objectives 1. Discovering the structure of the pathway by investigating if other bZIPs, in addition to bZIP63 contribute to the regulation of the circadian oscillator by sugars2. Identifying the molecular outputs from the pathway by determining how bZIP63 regulates the circadian clock through transcriptional analysis coupled to CHIPseq, molecular studies and investigation of protein-protein interactions3. Identifying the physiological outputs of the pathway by determining why bZIP63 (and potentially other bZIPs) regulate the circadian clock. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VIANA, AMERICO J. C.; MATIOLLI, CLEVERSON C.; NEWMAN, DAVID W.; VIEIRA, JOAO G. P.; DUARTE, GUSTAVO T.; MARTINS, MARINA C. M.; GILBAULT, ELODIE; HOTTA, CARLOS T.; CALDANA, CAMILA; VINCENTZ, MICHEL. he sugar-responsive circadian clock regulator bZIP63 modulates plant growt. NEW PHYTOLOGIST, v. 231, n. 5, p. 1875-1889, SEP 2021. (19/25993-9, 15/06260-0, 08/52071-0, 18/25710-4, 14/04117-2, 12/09351-8)

VIANA, AMERICO J. C.; MATIOLLI, CLEVERSON C.; NEWMAN, DAVID W.; VIEIRA, JOAO G. P.; DUARTE, GUSTAVO T.; MARTINS, MARINA C. M.; GILBAULT, ELODIE; HOTTA, CARLOS T.; CALDANA, CAMILA; VINCENTZ, MICHEL. The sugar-responsive circadian clock regulator bZIP63 modulates plant growth. NEW PHYTOLOGIST, JUN 2021. (12/09351-8, 19/25993-9, 18/25710-4, 15/06260-0, 14/04117-2, 08/52071-0)