Multi-User Equipment approved in grant 2017/21460-0: gas chromatography coupled with triple quadrupole tandem mass spectrometry (GC-MS/MS)

• Grant number: 19/13799-3
• Support Opportunities: Multi-user Equipment Program
• Duration: August 01, 2019 - July 31, 2026
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Renato Beozzo Bassanezi
• Grantee: Renato Beozzo Bassanezi
• Host Institution: Fundo de Defesa da Citricultura (FUNDECITRUS). Araraquara , SP, Brazil
• Associated research grant: 17/21460-0 - Measures for reducing primary infections in the integrated management of Huanglongbing in São Paulo citrus belt: technical and economic feasibility, AP.TEM
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Caracterização de Materiais - Análises Químicas - Cromatografia gasosa acoplada a espectrômetro de massa
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

The equipment GC-MS/MS approved by Fapesp as a multi-user equipment is a gas chromatography coupled with triple quadrupole tandem mass spectrometry. The gas chromatography (GC) is ideal for applications where is necessary to separate mixtures with high vapor pressure molecules, e.g. volatiles compounds. The injection of these compounds into the GC is possible both in the gas phase and diluted in solutions of organic solvents. After the separation, each compound in the GC is detected in the mass spectrometer (MS). The MS, through the electron impact ionization, allow identify and quantify the molecules. The MS is equipped with a tandem triplequadrupole analyzer, that allows to performing experiments as product ion, precursor ion, neutral loss, selected reaction monitoring (SRM) and other. The main advantages of these experiments are the support in compounds identification, specificity in analyses and sensibility in quantitative methods. The Fundecitrus recently acquired a thermal desorption equipment (TD) to support the activities in biotechnology and chemical ecology areas in our institution. The TD is a very versatile tool in the investigation of volatile organic compounds (VOCs) and semi-volatile organic compounds (SVOCs). In this technology, the volatile compounds emitted by plants, insects, ambient air, foods, beverages, polymeric material and from other matrices are adsorbed in commercial tubes filled with thermal stable adsorbents polymers. The TD is used to desorbed, under high temperatures, the VOCs and SVOCs previously trapped in the adsorbents polymers and inject straight to the GC, without the necessity of solubilizing the volatiles or eluted them from the adsorbent polymers with organic solvents. This procedure brings a series of advantages, as the transport of the samples desorbed in the commercial tubes, avoid the dilution effects, high sensibility and other. The TD equipment will be installed in the GC-MS/MS as a Fundecitrus counterpart to the research project that will turn, in this way, in a versatile tool in possibilities for in-let the samples that, consequently, will increase considerably the spectra of applications. The coupling of these two instruments will compose them in a TD-GC-MS/MS, a tool that the Fapesp multi-users laboratories does not have yet, and this equipment will be able to perform the thematic project experiments itself and for the users of this multi-users equipment. The Fundecitrus already undertakes to install the GC-MS/MS equipment following the manufacturer's recommendations, respecting the reasonable time that does not compromise the progress of the thematic project, will provide a laboratory assistant with bachelor's degree in chemistry that will be responsible for the operation of the equipment and, finally, in a place suitable for receiving the multiuser and under the supervision of a researcher expert in mass spectrometry. (AU)