Mulit-user equipment approved in grant 2017/21539-6:ODYSSEY CLX- licor

• Grant number: 19/18476-8
• Support Opportunities: Multi-user Equipment Program
• Start date: October 01, 2019
• End date: September 30, 2026
• Field of knowledge: Biological Sciences - Pharmacology
• Principal Investigator: Christiane Becari
• Grantee: Christiane Becari
• Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated research grant: 17/21539-6 - Role of renin angiotensin system in Abdominal Aortic Aneurysm, AP.JP
• EMU web page: Página do Equipamento Multiusuário não informada
• Type of equipment: Processos Biológicos - Caracterização - Infravermelho (IR); Processos Biológicos - Caracterização - Leitores de placas
• Manufacturer: Fabricante não informado
• Model: Modelo não informado

Abstract

Abdominal aortic aneurysm (AAA) is a progressive disease leading to vascular wall deterioration and, gradual aneurysm expansion and, eventually, rupture. The incidence and mortality of AAA are high. The treatment for AAA is the open conventional repair or the endovascular aneurysm repair (EVAR). There is no known effective AAA medical treatment that can inhibit its growth, development or the rupture risk in human beings. Partly, it is due to a lack of a more detailed knowledge about the genetic, cellular and humoral mechanisms in the AAA pathophysiology. The renin-angiotensin system (RAS) participates in several physiological and pathophysiology processes in both cardiovascular and renal systems. Experimental data show that continuous angiotensin II (AngII) infusion can induce AAA development in mice. There are also experimental and clinical evidences that the use of RAS inhibitors (angiotensin converting enzyme inhibitors- ACE inhibitors) and AT1 receptor (AT1R) antagonists can affect the aneurysm stability and reduce the rupture risk. Recently, new RAS modulation paradigms have emerged in which there might be two main RAS pathways: one ACE/Ang II/AT1R deleterious path and, a beneficial ACE-2/Ang 1-7/MASR. In addition, the biological role of other components such as Ang IV, Ang 1-9 has been characterized. Recently, the role of AT4 receptor-binding Ang IV was related as a protective factor to the development of AAA in mice. However, much of this data relating AAA and RAS peptides, including Ang II, Ang 1-7 and Ang IV, were performed on experimental animals. Little is known about the role of Ang 1-9 and Ang III, as well as on the enzymes responsible for the formation of these peptides in this context. Thus, an innovative proposal of the present research project is the investigation of the role of these important components of RAS in the development of AAA. As previously demonstrated, they can produce, degrade and regulate the different types of angiotensin. Other enzymes also participate in the generation of Ang II such as, human chymase and mice and rats elastase-2 (ELA-2). Chymase and ELA-2 contributes to the formation of Ang II in different vascular beds. Thus, as ELA-2 is an Ang II-forming enzyme in the vascular system, we intend to study the contribution this enzyme to the pathophysiology of AAA. We will use a translational approach through models in mice and surgical human samples. The infiltration of inflammatory cells and the rupture of the extracellular matrix are also involved in the formation and progression of AAA. Studies have shown that one of the mechanisms by which Ang II induces AAA is through the production and increase of the activity of the metalloproteinases (MMPs, especially MMP-2 and MMP-9) in the extracellular tissue. In addition, cytokine secretion and the accumulation of inflammatory cells in the arterial wall may contribute to the development of AAA.Therefore, the present project aims to evaluate the contribution of the components of RAS to the pathophysiology of AAA through biochemical, molecular and histological analyzes. We will use samples from patients treated on HCRP-USP and experimental models of mice (C57Bl / 6 and ELA-2 knockout). The novelty of our study is the investigation of the ELA-2 / chymase / ACE / AngII / AT1R, ACE-2 / Ang1-7 / MASR axes, the Ang4 / AT4R interaction and the contribution of ELA-2 to AAA pathophysiology. Our translational approach linking clinical phenotypes of AAA patients with molecular, biochemical and histological characterization strengthens this design and enables the development of new strategies to better understand AAA in both molecular and clinical-surgical bases. (AU)