Development of microfluidic device for liquid biopsy of circulating tumor cells

• Grant number: 19/13411-5
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2019
• End date: September 30, 2022
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Analytical Chemistry
• Principal Investigator: Valber de Albuquerque Pedrosa
• Grantee: Valber de Albuquerque Pedrosa
• Host Institution: Instituto de Biociências (IBB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil

Abstract

Circulating tumor cells (CTCs) are cells released by primary and/or metastatic tumors that, upon release, circulate through the bloodstream or lymphatic system to form metastases. The CTC analysis still remains a major challenge for the scientific community. CTCs hold the key to better understanding the biology of metastasis and may provide better information for noninvasively measuring the evolution of tumor genotypes during treatment and disease progression. Therefore, the development of new techniques to retain purer CTC, susceptible to a better cellular and molecular characterization, will allow a wide range of clinical applications, and may be useful in monitoring the response to treatment in a fast, invasive and easier way when compared to the traditional biopsy, and is therefore known as a liquid biopsy. Therefore, this project aims to build a microfluidic system of separation of CTCs in blood, with high selectivity and recovery index. Specifically, the objectives of this work will be: to design and manufacture a microfluidic chip to separate and capture CTCs using anti-epithelial cell adhesion-controlled immobilization (anti-EpCAM), and characterization and optimization of microfluidic chip efficiency and specificity capture using tumor cell lines. The research will have broad impacts on students of different educational levels, exposing them to exciting challenges in science and engineering that have a high relevance for our society. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PEDROSA, VALBER A.; CHEN, KANGFU; GEORGE, THOMAS J.; FAN, Z. HUGH. Gold Nanoparticle-Based Microfluidic Chips for Capture and Detection of Circulating Tumor Cells. BIOSENSORS-BASEL, v. 13, n. 7, p. 9-pg., 2023-07-01. (19/13411-5, 17/25937-6)

CRULHAS, BRUNO P.; BASSO, CAROLINE R.; CASTRO, GUSTAVO R.; PEDROSA, VALBER A.. Detection of Prostate Cancer Biomarker PCA3 by Using Aptasensors. Current Medicinal Chemistry, v. 29, n. 37, p. 8-pg., 2022-01-01. (19/13411-5)

LISZBINSKI, RAQUEL B.; ROMAGNOLI, GRAZIELA G.; GORGULHO, CAROLINA M.; BASSO, CAROLINE R.; PEDROSA, VALBER A.; KANENO, RAMON. Anti-EGFR-Coated Gold Nanoparticles In Vitro Carry 5-Fluorouracil to Colorectal Cancer Cells. MATERIALS, v. 13, n. 2, JAN 2 2020. (15/26729-2, 19/13411-5)

MAGRO, MASSIMILIANO; BARATELLA, DAVIDE; VENERANDO, ANDREA; NALOTTO, GIULIA; BASSO, CAROLINE R.; MOLINARI, SIMONE; SALVIULO, GABRIELLA; UGOLOTTI, JURI; PEDROSA, VALBER A.; VIANELLO, FABIO. Enzyme Immobilization on Maghemite Nanoparticles with Improved Catalytic Activity: An Electrochemical Study for Xanthine. MATERIALS, v. 13, n. 7, APR 2020. (19/20573-1, 19/13411-5)

BASSO, CAROLINE R.; CRUZ, TAIS F.; SILVA, BRUNA L.; PEDROSA, VALBER A.; ARAUJO JUNIOR, JOAO P.. A Methodology for Porcine Circovirus 2 (PCV-2) Quantification Based on Gold Nanoparticles. MATERIALS, v. 13, n. 5, MAR 2020. (19/13411-5)