Evaluation of the potential of the production of biopolymers by methylotrophic bacteria

• Grant number: 18/22790-7
• Support Opportunities: BIOTA-FAPESP Program - Regular Research Grants
• Start date: November 01, 2019
• End date: October 31, 2022
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: Elen Aquino Perpetuo
• Grantee: Elen Aquino Perpetuo
• Host Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil
• Associated researchers: Bruno Karolski ; Letícia Oliveira Bispo Cardoso ; Louise Hase Gracioso

Abstract

It is estimated that approximately 299 million tons of plastics were produced in 2013 and the progression is that this consumption will continue to increase until 2020. However, about 60% of what is produced are non-biodegradable plastics, which makes them persistent environmentally, affecting both the environment and humans. Given this scenario, and as an environmentally correct alternative, we can make use of biopolymers, which can be produced naturally by bacteria under specific growth conditions. Polyhydroxyalkanoates (PHAs), for example, are a class of biodegradable, biocompatible biopolymers that have a wide range of characteristics due to their monomers already dated in the literature and may serve as potential substitutes for conventional plastics. However, they are still not widespread in our daily lives, because they present a high cost of production and purification. This is mainly due to the substrate used, which in most cases are sugars, and even represent 30% of the final price of the product. In order to decrease the production price, the objective of this work is to produce biopolymers using methylotrophic bacteria isolated from contaminated environments, capable of consuming compounds containing only 1 carbon atom in its structure. In this sense, methane and methanol are cheaper substrates and present in abundance in the environment. In addition, methane is an important greenhouse gas and main component (~ 70%) of the natural gas released in the oil exploration. In this context, this work is linked to CEPID-RCGI (www.rcgi.poli.usp.br), making use of biotechnological processes for the mitigation of methane for the production of biopolymers. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BISPO CARDOSO, LETICIA OLIVEIRA; KAROLSKI, BRUNO; GRACIOSO, LOUISE HASE; OLLER DO NASCIMENTO, CLAUDIO AUGUSTO; PERPETUO, ELEN AQUINO. Increased P3HB Accumulation Capacity ofMethylorubrumsp. in Response to Discontinuous Methanol Addition. Applied Biochemistry and Biotechnology, v. 192, n. 3, JUL 2020. (17/17438-0, 18/22790-7)

GRACIOSO, LOUISE HASE; PENA-BAHAMONDE, JANIRE; KAROLSKI, BRUNO; BORREGO, BRUNA BACARO; PERPETUO, ELEN AQUINO; OLLER DO NASCIMENTO, CLAUDIO AUGUSTO; HASHIGUCHI, HIROKI; JULIANO, MARIA APARECIDA; ROBLES HERNANDEZ, FRANCISCO C.; RODRIGUES, DEBORA FRIGI. Copper mining bacteria: Converting toxic copper ions into a stable single-atom copper. SCIENCE ADVANCES, v. 7, n. 17, APR 2021. (15/18089-3, 18/22790-7, 13/11020-2, 13/50218-2, 18/13588-0)

CARVALHO DE JESUS, PRISCILA DA COSTA; MENDES, MARIA ANITA; PERPETUO, ELEN AQUINO; BASSO, THIAGO OLITTA; OLLER DO NASCIMENTO, CLAUDIO AUGUSTO. Extracellular carotenoid production and fatty acids profile of Parachlorella kessleri under increased CO2 concentrations. Journal of Biotechnology, v. 329, p. 151-159, MAR 10 2021. (18/22790-7, 14/50279-4)

GRACIOSO, LOUISE HASE; BELLAN, ALESSANDRA; KAROLSKI, BRUNO; BISPO CARDOSO, LETICIA OLIVEIRA; PERPETUO, ELEN AQUINO; OLLER DO NASCIMENTO, CLAUDIO AUGUSTO; GIUDICI, REINALDO; PIZZOCCHERO, VALENTINO; BASAGLIA, MARINA; MOROSINOTTO, TOMAS. Light excess stimulates Poly-beta-hydroxybutyrate yield in a mangrove-isolated strain of Synechocystis sp.. Bioresource Technology, v. 320, p. 7-pg., 2021-01-01. (17/17438-0, 18/10811-0, 14/50279-4, 18/22790-7)

GRACIOSO, LOUISE HASE; BELLAN, ALESSANDRA; KAROLSKI, BRUNO; BISPO CARDOSO, LETICIA OLIVEIRA; PERPETUO, ELEN AQUINO; OLLER DO NASCIMENTO, CLAUDIO AUGUSTO; GIUDICI, REINALDO; PIZZOCCHERO, VALENTINO; BASAGLIA, MARINA; MOROSINOTTO, TOMAS. Light excess stimulates Poly-beta-hydroxybutyrate yield in a mangrove-isolated strain of Synechocystis sp.. Bioresource Technology, v. 320, n. B, JAN 2021. (18/10811-0, 18/22790-7, 14/50279-4, 17/17438-0)

MALVAR, SARA; CARDOSO, LETICIA O. B.; KAROLSKI, BRUNO; PERPETUO, ELEN A.; CARMO, BRUNO S.; MENEGHINI, JULIO R.. A rheological approach to identify efficient biopolymer producing bacteria. Biotechnology and Bioengineering, v. 118, n. 2, p. 622-632, FEB 2021. (19/15754-7, 16/14337-5, 18/22790-7, 14/50279-4)

NUNES DA SILVA, ESTHER CECILIA; KAROLSKI, BRUNO; BISPO CARDOSO, LETICIA OLIVEIRA; GRACIOSO, LOUISE HASE; PERPETUO, ELEN AQUINO. Biosynthesis of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) by a new mangrove-isolated Methylopila oligotropha strain MCSUBH. POLYMER BULLETIN, v. N/A, p. 14-pg., 2022-06-15. (20/15230-5, 14/50279-4, 18/22790-7, 17/17438-0)

BISPO CARDOSO, LETICIA OLIVEIRA; KAROLSKI, BRUNO; GRACIOSO, LOUISE HASE; BORREGO, BRUNA BACARO; OLLER DO NASCIMENTO, CLAUDIO AUGUSTO; PERPETUO, ELEN AQUINO. Enrichment of Methylosinus-dominant consortia from mangroves for polyhydroxybutyrate (PHB) production. JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING, v. 10, n. 5, p. 9-pg., 2022-09-08. (18/22790-7, 14/50279-4)