Development of methods to study Haemonchus contortus and anthelmintic resistance to monepantel

• Grant number: 19/02967-2
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2019
• End date: May 31, 2022
• Field of knowledge: Biological Sciences - Parasitology - Helminthology of Parasites
• Principal Investigator: Simone Cristina Méo Niciura
• Grantee: Simone Cristina Méo Niciura
• Host Institution: Embrapa Pecuária Sudeste. Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA). Ministério da Agricultura, Pecuária e Abastecimento (Brasil). São Carlos , SP, Brazil
• Associated researchers: Alessandro Francisco Talamini Do Amarante ; Alessandro Pelegrine Minho ; Ana Carolina de Souza Chagas ; Luciana Correia de Almeida Regitano ; Magda Vieira Benavides ; Marcia Cristina de Sena Oliveira ; Sergio Novita Esteves
• Associated scholarship(s): 19/27829-1 - Development of methods to study Haemonchus contortus, BP.TT

Abstract

Major economic losses in sheep production are caused by gastrointestinal nematode parasites, mainly by Haemonchus contortus, e by resistance to the anthelmintics used in their control. Therefore, this study was designed to increase knowledge in monepantel anthelmintic resistance and for H. contortus control in sheep. Firstly, polymorphisms detected in a genome-wide study will be validated as molecular markers of H. contortus resistance to monepantel, to be used for early diagnosis and monitoring of resistance in parasite populations. Furthermore, an in vitro technique for culture of H. contortus from eggs and infective larvae to fertile adults producing viable descendants will be developed, aiming to replace methods that involve sheep hosts for crossing parasites resulting in the introgression of resistance genes, and also to make available the adult stage of this nematode for in vitro assays of new compounds or for alternative methods for parasite control, as gene edition and vaccine development. Thus, considering the worldwide problem of anthelmintic resistance, the results obtained in this project will contribute to extend the efficacy of available commercial anthelmintics, to detect molecular markers of resistance in H. contortus, and to develop alternative measures for worm control in sheep flocks. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

NICIURA, SIMONE CRISTINA MEO; BENAVIDES, MAGDA VIEIRA; OKINO, CINTIA HIROMI; IBELLI, ADRIANA MERCIA GUARATINI; MINHO, ALESSANDRO PELEGRINE; ESTEVES, SERGIO NOVITA; CHAGAS, ANA CAROLINA DE SOUZA. Genome-Wide Association Study for Haemonchus contortus Resistance in Morada Nova Sheep. PATHOGENS, v. 11, n. 8, p. 12-pg., 2022-08-01. (19/02967-2, 17/01626-1, 19/02929-3)

MEO NICIURA, SIMONE CRISTINA; OKINO, CINTIA HIROMI; NUCCI, ALESSANDRA DA SILVA; MALAGO JR, WILSON; BENAVIDES, MAGDA VIEIRA; ESTEVES, SERGIO NOVITA; DE SOUZA CHAGAS, ANA CAROLINA. Polymorphisms in exon 11 of the mptl-1 gene and monepantel resistance in Haemonchus contortus. Parasitology Research, v. 121, n. 12, p. 8-pg., 2022-10-04. (19/02967-2)

SIMONE CRISTINA MÉO NICIURA; ALESSANDRO PELEGRINE MINHO; JENNIFER MCINTYRE; MAGDA VIEIRA BENAVIDES; CINTIA HIROMI OKINO; SERGIO NOVITA ESTEVES; ANA CAROLINA DE SOUZA CHAGAS; ALESSANDRO FRANCISCO TALAMINI DO AMARANTE. Cultivo in vitro de estádios parasitários de Haemonchus contortus. Revista Brasileira de Parasitologia Veterinária, v. 32, n. 1, 2023-01-13. (19/02967-2)

NICIURA, SIMONE CRISTINA MEO; MINHO, ALESSANDRO PELEGRINE; MCINTYRE, JENNIFER; BENAVIDES, MAGDA VIEIRA; OKINO, CINTIA HIROMI; ESTEVES, SERGIO NOVITA; CHAGAS, ANA CAROLINA DE SOUZA; DO AMARANTE, ALESSANDRO FRANCISCO TALAMINI. In vitro culture of parasitic stages of Haemonchus contortus. REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA, v. 32, n. 1, p. 12-pg., 2023-01-01. (19/02967-2)