The use of molecular cytogenetics for evolutioonary and taxonomic studies in Cervidae

• Grant number: 19/06940-1
• Support Opportunities: Regular Research Grants
• Start date: January 01, 2020
• End date: June 30, 2022
• Field of knowledge: Biological Sciences - Genetics - Animal Genetics
• Agreement: Czech Science Foundation (GACR)
• Principal Investigator: José Maurício Barbanti Duarte
• Grantee: José Maurício Barbanti Duarte
• Principal researcher abroad: Miluse Vozdova
• Institution abroad: Veterinary Research Institute (VRI), Czech Republic
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated researchers: Agda Maria Bernegossi ; David Javier Galindo Huamán ; Eluzai Dinai Pinto Sandoval
• Associated scholarship(s): 21/01653-4 - Assistance in obtaining chromosomal preparations for use in molecular cytogenetics and organization of the cell data bank, BP.TT

Abstract

The family Cervidae groups together species with a growing economic potential and endangered species. Karyotypes and phylogenetic position of many deer species are uncertain due to the lack of detailed scientific data. The extensive variation in chromosome numbers (2n=6-70) and structure makes this family suited for evolutionary studies based on interspecies molecular cytogenetic comparisons. In this project, we will perform a comparative analysis in a wide range of deer species, focusing on species lacking comprehensive data, including the genus Mazama and its cytotypes. We will determine karyotype differences and their impact on production of aneuploid spermatozoa and reproductive isolation, identify intrachromosomal rearrangements of the X chromosomes, and compare sequence similarities and chromosomal positions of satellite DNAs in various deer species. For this purpose, whole chromosome, BAC and satellite DNA probes for FISH will be constructed in VRI laboratory. Obtained data will be used to improve taxonomic classification, and review phylogenetic relationships within Cervidae. (AU)

Scientific publications

(7)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BERNEGOSSI, AGDA MARIA; DE SOUZA BORGES, CAROLINA HELOISA; PINTO SANDOVAL, ELUZAI DINAI; CARTES, JOSE LUIS; CERNOHORSKA, HALINA; KUBICKOVA, SVATAVA; VOZDOVA, MILUSE; CAPARROZ, RENATO; GONZALEZ, SUSANA; BARBANTI DUARTE, JOSE MAURICIO. Resurrection of the genus Subulo Smith, 1827 for the gray brocket deer, with designation of a neotype. JOURNAL OF MAMMALOGY, v. N/A, p. 15-pg., 2022-09-19. (17/07014-8, 19/06940-1)

SANDOVAL, ELUZAI DINAI PINTO; JEDRZEJEWSKI, WLODZIMIERZ; MOLINARI, JESUS; VOZDOVA, MILUSE; CERNOHORSKA, HALINA; KUBICKOVA, SVATAVA; BERNEGOSSI, AGDA MARIA; CAPARROZ, RENATO; DUARTE, JOSE MAURICIO BARBANTI. Description of Bisbalus, a New Genus for the Gray Brocket, Mazama cita Osgood, 1912 (Mammalia, Cervidae), as a Step to Solve the Neotropical Deer Puzzle. TAXONOMY, v. 4, n. 1, p. 17-pg., 2024-03-01. (19/06940-1, 17/07014-8)

SANDOVAL, ELUZAI DINAI PINTO; VACARI, GABRIELLE QUEIROZ; JULIA, JUAN PABLO; GONZALEZ, SUSANA; VOZDOVA, MILUSE; CERNOHORSKA, HALINA; KUBICKOVA, SVATAVA; KALTHOFF, DANIELA C.; DUARTE, JOSE MAURICIO BARBANTI. Assessing the Taxonomic Status of the Gray Brocket Mazama simplicicornis argentina Lonnberg, 1919 (Artiodactyla: Cervidae). ZOOLOGICAL STUDIES, v. 62, p. 11-pg., 2023-07-12. (17/07014-8, 19/06940-1)

BERNEGOSSI, AGDA MARIA; VOZDOVA, MILUSE; CERNOHORSKA, HALINA; KUBICKOVA, SVATAVA; GALINDO, DAVID JAVIER; KADLCIKOVA, DITA; RUBES, JIRI; BARBANTI DUARTE, JOSE MAURICIO. Cytogenetic Mapping of Cattle BAC Probes for the Hypothetical Ancestral Karyotype of the Family Cervidae. Cytogenetic and Genome Research, v. N/A, p. 8-pg., 2022-08-18. (17/07014-8, 19/20810-3, 19/06940-1, 18/25769-9)

MORALES-DONOSO, JORGE ALFONSO; VACARI, GABRIELLE QUEIROZ; BERNEGOSSI, AGDA MARIA; PINTO SANDOVAL, ELUZAI DINAI; FARIA PERES, PEDRO HENRIQUE; GALINDO, DAVID JAVIER; DE THOISY, BENOIT; VOZDOVA, MILUSE; KUBICKOVA, SVATAVA; BARBANTI DUARTE, JOSE MAURICIO. Revalidation of Passalites Gloger, 1841 for the Amazon brown brocket deer P. nemorivagus (Cuvier, 1817) (Mammalia, Artiodactyla, Cervidae). ZOOKEYS, v. N/A, n. 1167, p. 24-pg., 2023-06-14. (17/07014-8, 19/06940-1)

GALINDO, DAVID JAVIER; MARTINS, GABRIELA SIQUEIRA; VOZDOVA, MILUSE; CERNOHORSKA, HALINA; KUBICKOVA, SVATAVA; BERNEGOSSI, AGDA MARIA; KADLCIKOVA, DITA; RUBES, JIRI; DUARTE, JOSE MAURICIO BARBANTI. Chromosomal Polymorphism and Speciation: The Case of the Genus Mazama (Cetartiodactyla; Cervidae). GENES, v. 12, n. 2, FEB 2021. (17/07014-8, 10/50748-3, 19/06940-1)

VOZDOVA, MILUSE; KUBICKOVA, SVATAVA; MARTINKOVA, NATALIA; GALINDO, DAVID JAVIER; BERNEGOSSI, AGDA MARIA; CERNOHORSKA, HALINA; KADLCIKOVA, DITA; MUSILOVA, PETRA; DUARTE, JOSE MAURICIO; RUBES, JIRI. Satellite DNA in Neotropical Deer Species. GENES, v. 12, n. 1, JAN 2021. (19/06940-1)