Lipid metabolism of bijupirá, Rachycentron canadum juveniles during the postprandial period and using alternative fatty acid sources

• Grant number: 19/26018-0
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2020
• End date: February 28, 2023
• Field of knowledge: Agronomical Sciences - Fishery Resources and Fishery Engineering - Aquaculture
• Principal Investigator: Renata Guimarães Moreira Whitton
• Grantee: Renata Guimarães Moreira Whitton
• Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated researchers: Alexandre Wagner Silva Hilsdorf ; Bruno Cavalheiro Araújo ; Giovana Souza Branco ; Paulo Henrique de Mello ; Renato Massaaki Honji

Abstract

The use of fish oil (FO) in marine aquaculture is important because of the need for long-chain and highly unsaturated fatty acids (LC-PUFA) from the n-3 series. However, the production of FO is limited, and alternative sources of lower cost and more stable production such as vegetable oils (VO) have been studied. However, this substitution, in most cases, negatively impacts growth and alters lipid metabolism, mainly due to the high amounts of saturated n-6 series (PUFAn-6), monounsaturated (MUFA) and polyunsaturated FA, and inversely to the absence of LC-PUFAs. An alternative for producing lower cost diets that meets nutritional needs is the use of VO supplemented with LC-PUFAs. However, studies with marine fish have shown that diets with high amounts of SFA, MUFAs and n-6 PUFA, even supplemented with LC-PUFA, alter lipid deposition in the liver and intestine as well as the expression of genes related to the synthesis and oxidation of AGs. Based on this problem, the objectives of this project are: 1) to understand initially the metabolic responses of Rachycentron canadum (bijupirá) juveniles in the postprandial period; 2) to investigate the effects of the use of alternative lipid sources supplemented with the main LC-PUFA on the performance and lipid metabolism of this species. The influence of different ratios of SFA/MUFA and C18 PUFAn-6/LC-PUFA on diets without FO will be evaluated. Growth parameters, FA profile of lipolytic and lipogenic tissues, liver and intestinal morphology, as well as the expression of genes related to the synthesis and oxidation pathways of AGs will be evaluated. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ARAUJO, BRUNO C.; BARBOSA, DAVID ACIOLE; HONJI, RENATO M.; BRANCO, GIOVANA S.; MENEGIDIO, FABIANO B.; MARQUES, VICTOR H.; MOREIRA, RENATA G.; KITAHARA, MARCELO V.; ROMBENSO, ARTUR N.; DE MELLO, PAULO H.; et al. Post-feeding Molecular Responses of Cobia (Rachycentron canadum): RNA-Sequencing as a Tool to Evaluate Postprandial Effects in Hepatic Lipid Metabolism. MARINE BIOTECHNOLOGY, v. 25, n. 3, p. 14-pg., 2023-05-10. (19/26018-0, 16/12435-0)

BARBOSA, DAVID ACIOLE; ARAUJO, BRUNO C.; BRANCO, GIOVANA SOUZA; SIMEONE, ALEXANDRE S.; HILSDORF, ALEXANDRE W. S.; JABES, DANIELA L.; NUNES, LUIZ R.; MOREIRA, RENATA G.; MENEGIDIO, FABIANO B.. Transcriptomic Profiling and Microsatellite Identification in Cobia (Rachycentron canadum), Using High-Throughput RNA Sequencing. MARINE BIOTECHNOLOGY, DEC 2021. (19/26018-0)