Membrane of collagen extracted from marine sponges for applications in skin burns

• Grant number: 19/15848-1
• Support Opportunities: Research Grants - Innovative Research in Small Business - PIPE
• Start date: December 01, 2020
• End date: August 31, 2021
• Field of knowledge: Health Sciences - Collective Health - Public Health
• Principal Investigator: Tiago Akira Tashiro de Araujo
• Grantee: Tiago Akira Tashiro de Araujo
• Company: Osteovita Biotecnologia Eireli
• CNAE: Atividades de atenção à saúde humana não especificadas anteriormente
• City: Campinas
• Pesquisadores principais: Antonio Carlos da Silva
• Associated researchers: Hananiah Tardivo Quintana ; Julia Risso Parisi ; Matheus de Almeida Cruz
• Associated scholarship(s): 20/15594-7 - Membrane of collagen extracted from marine sponges for applications in skin burns, BP.PIPE

Abstract

Burns represent a serious public health problem, leading to a series of complications and sequelae for the affected individuals, with a significant reduction in their quality of life. This fact is aggravated by taking into account the high cost caused by the treatment required to the Unified Health System (SUS). For burns of greater extent there is a need for hospitalization to perform surgical interventions, debridements, among others. In addition, another intervention of choice is the placement of cutaneous membranes in the region of the lesion, in order to protect the area, avoiding infections and integrating with the tissues, promoting their regeneration. One of the materials most used for the manufacture of membranes is collagen (bovine or porcine origin). These allow the control of pain through the occlusion of nerve endings and facilitate the migration of epithelial cells and healing. Another great advantage of this type of intervention is that it is resorbable and there is no need to change dressings during the repair period. However, these membranes present a high cost as well as great risk of transmission of pathogens. In an attempt to solve this problem, the company Osteovita Biotecnologia developed a cutaneous membrane manufactured from collagen extracted from marine sponges. The species used (Chondrilla caribensis species), widely available on the Brazilian coast, is known to be rich in collagen (similar to collagen type XIII and amino acid composition similar to vertebrate collagen). Thus, the company already has expertise to perform the extraction of collagen and has already conducted a series of studies showing the biocompatibility, lack of genotoxicity and cytotoxicity and adequate biological performance of marine collagen. From this promising raw material we manufactured the collagen membrane to be used in burns. It should be noted that said membrane has a microfibrillar structure, with different thicknesses, easy handling, mechanical and tensile strength and elasticity. Thus, the present proposal aims to characterize and evaluate the cytotoxicity and genotoxicity of this marine collagen membrane (through in vitro studies). The results will provide relevant information regarding morphology, physico-chemical properties, as well as biological potential (ability to stimulate cells and compatibility) of the same. These data, in turn, are of paramount importance for initial evaluation of the material, and will provide support for pre-clinical (in vivo) testing as well as clinical trials. In this way, it is intended to supply part of the biomembrane market (which is in rapid expansion), offering a membrane manufactured from raw material extremely effective to accelerate tissue growth, in addition to being extremely biologically safe and presenting a price accessible end. (AU)