3D bioprinted scaffolds from biosilica of marine sponges for treatment of bone fractures and bone defects

• Grant number: 20/00352-8
• Support type: Regular Research Grants
• Duration: September 01, 2020 - August 31, 2022
• Field of knowledge: Interdisciplinary Subjects
• Principal researcher: Ana Claudia Muniz Renno
• Grantee: Ana Claudia Muniz Renno
• Home Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil
• Assoc. researchers: Matheus de Almeida Cruz ; Renata Neves Granito ; Tiago Akira Tashiro de Araujo ; Veerle Bloemen
• Associated scholarship(s): 22/00485-3 - 3D bioprinted scaffolds from biosilica of marine sponges for treatment of bone fractures and bone defects, BP.TT; 21/06399-9 - 3D bioprinted scaffolds from biosilica of marine sponges for treatment of bone fractures and bone defects, BP.TT; 20/13038-0 - 3D bioprinted scaffolds from biosilica of marine sponges for treatment of bone fractures and bone defects, BP.TT; 20/13040-4 - 3D bioprinted scaffolds from biosilica of marine sponges for treatment of bone fractures and bone defects, BP.TT

Abstract

There is a growing need for grafts to treat bone loss caused by trauma or diseases in Brazil. However, the vast majority of synthetic biomaterials developed for this purpose is imported or dependent upon foreign technology, which limits the spread of such products in medical practice, particularly in the public health system. Besides being economically affordable, natural grafts are also considered more biocompatible than synthetic biomaterials because they have a bio-interactive surface for cell adhesion and proliferation. Within this context, this study aims to take advantage of the great potential of Brazilian marine biodiversity and assess the biological performance of marine sponges easily found in the country's coast, with the purpose of using them as bone substitutes in tissue engineering. Therefore, the objectives of this study are: (i) to produce and characterize new 3D bioprinted scaffolds manufactured from biosilica extracted from marine sponges and to assess, through in vivo tests, the fibrous scaffolds manufactured from biosilica and collagen extracted from marine sponges, using a calvarial bone defect model in rats. For this purpose, the composites will characterize by scanning electron microscope (SEM) and Fourier transform infrared spectroscopy (FTIR). For the in vivo test will be used these experimental groups: Control and 3D bioprinted scaffolds manufactured from biosilica of marine sponges. For the in vivo tests, 48 Wistar rats will be submitted to a surgical procedure in order to induce a calvarial bone defect model. The defect will be filled with the 3D scaffolds or non-treatment. Thus, each experimental group will be divided into 2 subgroups, euthanized after 15 and 45 days post-surgery. It will be performed of histopathology, histomorphometry, micro-CT and immunohistochemistry analysis. These studies will provide data regarding the structural properties and the osteogenic potential of these marine sponges, which will be valuable for the development of high effective biomaterials, in terms of quality and accessibility, for the treatment of bone dysfunction or disease. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

BONIFACIO, MIRIAN; BENFATO, IZABELLE DIAS; DE ALMEIDA CRUZ, MATHEUS; DE SALES, DANIELE CORREIA; PANDOLFO, ISABELLA LIBA; QUINTANA, HANANIAH TARDIVO; CARVALHO, CAROLINA PRADO DE FRANCA; DE OLIVEIRA, CAMILA APARECIDA MACHADO; RENNO, ANA CLAUDIA MUNIZ. Effects of photobiomodulation on glucose homeostasis and morphometric parameters in pancreatic islets of diabetic mice. Lasers in Medical Science, OCT 2021. Web of Science Citations: 0.