Additive manufacturing applied in the development a new bioglass scaffold of the SiO2 - Na2O - CaO - P2O5 system, developed from marine sponge biosilica: biological evaluation in vitro and in vivo

• Grant number: 22/13535-9
• Support Opportunities: Scholarships in Brazil - Master
• Start date: April 01, 2023
• End date: September 30, 2025
• Field of knowledge: Interdisciplinary Subjects
• Principal Investigator: Renata Neves Granito
• Grantee: Amanda Sardeli Alqualo Assaf
• Host Institution: Instituto de Saúde e Sociedade (ISS). Universidade Federal de São Paulo (UNIFESP). Campus Baixada Santista. Santos , SP, Brazil
• Associated scholarship(s): 24/04270-7 - The analysis of gelatin methacryloyl hydrogel constructs loaded with chondrocytes in the regeneration of osteochondral tissue in the knee joint., BE.EP.MS

Abstract

With the increase in cases of bone fractures and the difficulty of regenerating critical defects,the tissue engineering has been applied to the development of new therapeutic interventions.The richbiological diversity of marine ecosystems makes them abundant in bioactive compounds favorable to thedevelopment of innovative biotechnological products. Several studies have shown that the biosilica extractedfrom marine sponges has osteogenic properties. In this context, this proposal aims to take advantage of thehuge potential for the development of additive manufacturing (3D-printed) scaffold of a new bioglass fromthe SiO2 - Na2O - CaO - P2O5 system, using biosilica from marine sponges., with in vitro and in vivoevaluation. The project will go through 4 stages: (1) the collection of marine sponges with the extraction ofbiosílica and synthesis of the new bioglass; (2) the development of the 3D-printed scaffolds; (3) in vitrostudies involving direct tests with osteoblast precursor cells to assess the adherence and proliferation; (4) invivo studies with the surgical implantation of the 3D printed scaffolds in bone defects in the skullcap of malerats aged 3 months and weighing between 250 and 300 grams. Thus, the results of these studies will providedata on the processing and the preclinical osteogenic potential of a biomaterial, which will be valuable forthe development of highly effective biomaterials, in terms of quality and accessibility to the citizen, for thetreatment of bone tissue disorders from the sustainable use of marine biodiversity of a planet called "blue".