Development of an innovative strategy for in-office tooth bleaching: aesthetic effectivity and biocompatibility associated with reduced clinical time

• Grant number: 20/09095-8
• Support Opportunities: Regular Research Grants
• Start date: December 01, 2020
• End date: November 30, 2022
• Field of knowledge: Health Sciences - Dentistry - Dental Materials
• Principal Investigator: Carlos Alberto de Souza Costa
• Grantee: Carlos Alberto de Souza Costa
• Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
• Associated researchers: Diana Gabriela Soares dos Passos ; Josimeri Hebling Costa

Abstract

Despite the excellent aesthetic outcome obtained using the in-office dental bleaching therapy, which uses gels with high concentrations of hydrogen peroxide (H2O2), this professional treatment may cause irreversible pulp damage and tooth sensitivity. Thus, new dental bleaching protocols based on the use of gels with low concentrations of H2O2 or shortened periods of gels application on enamel have been assessed. However, these new tooth bleaching protocols have negatively influenced the aesthetic outcome or request a number of bleaching sessions, turning impracticable their clinical application. Therefore, innovative in-office tooth bleaching strategies capable of getting fast and appropriate aesthetic results with no pulp damage or post-treatment sensitivity are needed. The aim of the present study is to apply the knowledges of Biomaterials Engineering and Advanced Oxidative Processes to develop a Universal Protective Barrier (UPB), that will recover dental enamel before applying the bleaching gel which will be irradiated or not with violet LED. In Phase 1, standardized enamel/dentin discs will be obtained, stained and distributed into groups; these discs will be used in the following phases of this project. In Phase 2, a Protective Polymeric Tape (PPT) and a Catalytic Primer (CP) will be developed to co-work as a double barrier (UPB) to improve the in-office tooth bleaching. In Phase 3, after covering the enamel with the UPB, the bleaching gel with 35% H2O2 will be applied on it for 45 min. and the aesthetic outcome as well as and trans-enamel and trans-dentinal toxicity on the odontoblast-like MDPC-23 cells will be assessed. In Phase 4, after covering the enamel with the UPB, the bleaching gel with 35% H2O2 will be applied for 15, 30 and 45 min. on it and irradiated with violet LED. Then, the aesthetic outcome as well as and trans-enamel and trans-dentinal toxicity on the MDPC-23 cells will be assessed. The shorter tooth bleaching time strategy that provides an aesthetic outcome like the traditional in-office tooth bleaching therapy will be selected. In Phase 5, the tooth bleaching strategy selected in Phase 4 will be assessed concerning its cytocompatibility using a 3D model of human dental pulp cells. All data obtained in this in vitro study will be submitted to specific statistical analysis. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PANSANI, TAISA NOGUEIRA; BASSO, FERNANDA GONCALVES; CARDOSO, LAIS MEDEIROS; COSTA, CARLOS ALBERTO DE SOUZA. EGF coating of titanium surfaces modulates cytokines in oral mucosal primary cells exposed to TNF-alpha. JOURNAL OF PERIODONTAL RESEARCH, v. 58, n. 4, p. 9-pg., 2023-05-24. (20/09095-8, 19/20783-6)

DIANA GABRIELA SOARES; NANCY TOMOKO SACONO; ANA PAULA DIAS RIBEIRO; MARIA LUISA LEITE; CARLA CAROLINE DE OLIVEIRA DUQUE; MARJORIE DE OLIVEIRA GALLINARI; LEANDRO EDGAR PACHECO; JOSIMERI HEBLING; CARLOS ALBERTO DE SOUZA COSTA. Pro-inflammatory mediators expression by pulp cells following tooth whitening on restored enamel surface. Brazilian Dental Journal, v. 33, n. 2, p. 83-90, 2022-04-29. (20/09095-8)

LEITE, MARIA LUISA; ANSELMI, CAROLINE; SOARES, IGOR PAULINO MENDES; MANSO, ADRIANA PIGOZZO; HEBLING, JOSIMERI; CARVALHO, RICARDO MARINS; COSTA, CARLOS ALBERTO DE SOUZA. Calcium silicate-coated porous chitosan scaffold as a cell-free tissue engineering system for direct pulp capping. Dental Materials, v. 38, n. 11, p. 14-pg., 2022-10-14. (20/09095-8, 19/14965-4)

RIBEIRO, RAFAEL ANTONIO DE OLIVEIRA; MARTINS, BEATRIZ VOSS; DIAS, MARLON FERREIRA; PERUCHI, VICTORIA; SOARES, IGOR PAULINO MENDES; ANSELMI, CAROLINE; HEBLING, JOSIMERI; COSTA, CARLOS ALBERTO DE SOUZA. Catalysis-based approaches with biopolymers and violet LED to improve in-office dental bleaching. Lasers in Medical Science, v. 39, n. 1, p. 10-pg., 2024-01-02. (20/09095-8, 21/01184-4)

RIBEIRO, RAFAEL ANTONIO DE OLIVEIRA; PERUCHI, VICTORIA; FERNANDES, LIDIA DE OLIVEIRA; ANSELMI, CAROLINE; SOARES, IGOR PAULINO MENDES; HEBLING, JOSIMERI; COSTA, CARLOS ALBERTO DE SOUZA. The influence of violet LED application time on the esthetic efficacy and cytotoxicity of a 35% H2O2 bleaching gel. Photodiagnosis and Photodynamic Therapy, v. 40, p. 6-pg., 2022-12-01. (20/09095-8)