Evaluation of microorganisms present in peritoneal fluid in patients with deep endometriosis in the etiopathogenesis of the disease

• Grant number: 19/24351-3
• Support Opportunities: Regular Research Grants
• Start date: February 01, 2021
• End date: January 31, 2024
• Field of knowledge: Health Sciences - Medicine - Maternal and Child Health
• Principal Investigator: Sérgio Podgaec
• Grantee: Sérgio Podgaec
• Host Institution: Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE). São Paulo , SP, Brazil
• City of the host institution: São Paulo
• Associated researchers: Bruna Cestari de Azevedo ; Camila Hernandes Pinheiro ; Helena Malvezzi ; Luiz Vicente Rizzo

Abstract

Endometriosis is a chronic disease that affects approximately 10-15% of women of reproductive age and that causes great impact on the quality of life of the patients. The complexity of the disease makes the mechanisms involved in its etiopathogenesis not yet fully elucidated. Recently, research has proposed an involvement of microorganisms in the pathogenesis of the disease, and the involvement of these microorganisms with the inflammasomes. The objective of this work is to prove that the microorganisms present in the peritoneal fluid of patients with endometriosis are responsible for the exaggerated activation of inflammasomes, leading to the development and maintenance of the disease. The microbiome profile and the analysis of 84 genes related to endometrial inflammasomes, lesions of deep retrocervical endometriosis and peritoneal fluid of patients with endometriosis, and of patients without endometriosis will be performed using genetic sequencing techniques and PCR Array. In endometrial cell cultures of patients with endometriosis and patients without endometriosis, the treatment with the peritoneal fluids will be analyzed through the microbiome and inflammasomes analyzes, in order to show the mechanism of action of these microorganisms present in the peritoneal fluid in the facility and maintenance of endometriosis, through the activation of inflammasomes (AU)