Development of bilaminary dermal matrix of bovine collagen impregnated with bacteriostatic agent

• Grant number: 19/22977-2
• Support Opportunities: Research Grants - Innovative Research in Small Business - PIPE
• Duration: July 01, 2021 - February 28, 2022
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Alexandre Graná Pinto
• Grantee: Alexandre Graná Pinto
• Host Company: MLA Suprimentos Médicos Ltda
• CNAE: Fabricação de instrumentos e materiais para uso médico e odontológico e de artigos ópticos ; Comércio atacadista de instrumentos e materiais para uso médico, cirúrgico, ortopédico e odontológico
• City: Jundiaí
• Associated researchers: Daniel Komatsu ; Eliana Aparecida de Rezende Duek ; Julio Henrique Filipini Rosa ; Renata Timm da Silva Oliveira
• Associated grant(s): 22/04726-5 - Development of bilaminar dermal matrix of bovine collagen impregnated with bacteriostatic agent, AP.PIPE

Abstract

The skin is an important organ of the human body and acts as the body's first defense system, being directly involved in the protection against external agents (Goodarzi et al., 2018). Thus, the loss of the skin barrier predisposes to infections, increased insensitive loss of water and changes in thermal regulation, causing increased morbidity, prolonged hospitalizations with high cost and even causing the death of the individual. Thus, there is currently much interest in synthetic or biological materials that can be used in the manufacture of dermal regeneration matrices. These have been consolidating as an effective alternative in the treatment of complex skin lesions, due to their versatility of application, reducing treatment and hospitalization times and, consequently, hospital costs (Shahrokhi, 2014).In Brazil, there is a growing demand for dermal matrices that favor the recovery of skin lesions, which may originate from surgical procedures, such as breast reconstruction, or due to some type of trauma, such as the burns (Jones, 2002; Frame, 2004). It is important to mention that in dermal matrices the physicochemical, biochemical and mechanical characteristics must also be developed in such a way that such devices are easily manageable during their implantation and can be perfectly accommodated over the lesion bed. Thus, the essential characteristics of the applicability of dermal matrices are: biocompatibility, prevention of water loss, acting as a barrier to bacterial invasion, possibility of being applied in a single surgical act, flexibility, market availability and low cost. In addition, it should not generate an exacerbated inflammatory response and should not stimulate the development of pathological scars. Therefore, the desired characteristics for such a product, aiming at dermal regeneration, are many and bringing them together in one device is still an open challenge. Currently, what exists on the world market are several skin substitutes, the most used being dermal regeneration matrices, which may consist of a bovine collagen layer, with or without elastin, covered or not with a silicone membrane. Therefore, the design challenge is to optimize the design of this type of dermal matrix so that the inner lamina, which will be in direct contact with the injured region, is made up of collagen and the outer lamina composed of silicone. In this way, the collagen slide will act as a framework, allowing cell migration and also extracellular matrix deposition, similar to the human dermis. The silicon blade, in turn, will act as a barrier against fluid loss and microorganism entry. As a differentiator, the incorporation of triclosan, a bacteriostatic agent widely used by industry, aims at greater efficiency in the protection of bacteria in order to improve the regeneration process against currently available dermal matrices, since one of the reasons for matrix loss dermal, after placing it on the lesion, is precisely the susceptibility to infections to which they are subjected (Quezada, 2009; Pharm C, 2007; Balasubramani 2001; Rhodes, 2004). (AU)