Abiotic factors and pregestational obesity: repercussions of leptin, melatonin and cortisol on human colostrum

• Grant number: 19/25112-2
• Support Opportunities: Regular Research Grants
• Start date: April 01, 2021
• End date: March 31, 2023
• Field of knowledge: Health Sciences - Collective Health - Public Health
• Principal Investigator: Luiz Carlos de Abreu
• Grantee: Luiz Carlos de Abreu
• Host Institution: Centro Universitário FMABC (FMABC). Santo André , SP, Brazil
• Associated researchers: Adenilda Cristina Honorio França ; Blanca Elena Guerrero Daboin ; Carlos Eduardo Gomes Siqueira ; Danny Laura Gomes Fagundes ; Eduardo Luzía França ; Fernando Adami ; Fernando Luiz Affonso Fonseca ; Khalifa Elmusharaf ; Ocilma Barros de Quental ; Patricia Fernandes Massmann ; Tassiane Cristina Morais

Abstract

It is possible that maternal obesity entails changes in hormonal components of human colostrum and in the imbalance between Th17 and Treg cells, modifying T lymphocyte homeostasis and thus the plasticity of macrophages that are important regulators of the inflammatory response, impacting in the increased risk of infant infection. However, so far, it is not known how the colostrum constituents of women who had a pregnancy implicated by obesity influence the polarization of human macrophages to M1 (proinflammatory) and M2 (antiinflammatory) phenotypes. Therefore, the objective of this research is to evaluate the effects of leptin, melatonin, cortisol and T cells on plasticity and polarization of colostrum macrophages of women with pregnancy complicated by obesity, as well as the influence of abiotic factors between different continents. A cross-sectional study will be conducted with laboratory analysis of 120 women from 3 different geographical locations (40 from Brazil, 40 from the United States of America, 40 from Ireland), considering maternal body mass index (BMI) and abiotic differences. Colostrum will be divided into two groups: control group (BMI between 18.5 to 24.9 kg/m2) and obesity (BMIe30 kg/m2). Cell populations will be obtained by magnetic beads. The detection of macrophage phenotypes and balance between Th17/Treg cells will be performed by flow cytometry. Colostrum hormones, levels of their respective cell receptors, and intracellular markers of macrophage phenotypes will be performed by ELISA. Significant differences will be considered when p <0.05. The findings of this study will bring more evidence to understand the impacts of abiotic factors on chronobiology, which associated with obesity may cause changes in the colostrum constituents that is fundamental for the modulation of the immune system of newborns and the nutritional status of infants. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DO AMARAL VIRGINIO PEREIRA, GABRIELLE; MORAIS, TASSIANE CRISTINA; FRANCA, EDUARDO LUZIA; GUERRERO DABOIN, BLANCA ELENA; PINHEIRO BEZERRA, ITALLA MARIA; PESSOA, RAFAEL SOUZA; DE QUENTAL, OCILMA BARROS; HONORIO-FRANCA, ADENILDA CRISTINA; DE ABREU, LUIZ CARLOS. Leptin, Adiponectin, and Melatonin Modulate Colostrum Lymphocytes in Mothers with Obesity. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 24, n. 3, p. 14-pg., 2023-02-01. (19/25112-2)

SILVA, KENIA MARIA REZENDE; FRANCA, DANIELLE CRISTINA HONORIO; DE QUEIROZ, ADRIELE ATAIDES; FAGUNDES-TRICHES, DANNY LAURA GOMES; DE MARCHI, PATRICIA GELLI FERES; MORAIS, TASSIANE CRISTINA; HONORIO-FRANCA, ADENILDA CRISTINA; FRANCA, EDUARDO LUZIA. Polarization of Melatonin-Modulated Colostrum Macrophages in the Presence of Breast Tumor Cell Lines. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 24, n. 15, p. 15-pg., 2023-08-01. (19/25112-2)

HONORIO-FRANCA, ADENILDA CRISTINA; DE ABREU, LUIS CARLOS; ELMUSHARAF, KHALIFA; SIQUEIRA, EDUARDO; FRANCA, EDUARDO LUZIA. Editorial: Breast milk: From nutrition to immunological action. FRONTIERS IN IMMUNOLOGY, v. 13, p. 3-pg., 2022-10-18. (19/25112-2)