Fast development of single-domain antibodies for research

• Grant number: 20/13229-0
• Support Opportunities: Research Grants - Innovative Research in Small Business - PIPE
• Duration: October 01, 2021 - June 30, 2022
• Field of knowledge: Biological Sciences - Biophysics - Molecular Biophysics
• Principal Investigator: Paulo Henrique Conaggin Godoi
• Grantee: Paulo Henrique Conaggin Godoi
• Host Company: PHC Godoi Biotecnologia
• CNAE: Pesquisa e desenvolvimento experimental em ciências físicas e naturais
• City: São Carlos
• Associated scholarship(s): 21/12528-6 - Expeditious development of single-chain antibodies for scientific research, BP.PIPE

Abstract

Single chain antibodies are versatile molecules that combine the high affinity and selectivity properties of typical antibodies and properties of much smaller molecules, having greater structural stability and solubility, combined with a simplicity in production, lower development costs and flexibility for genetic manipulation. This proposal aims to explore these characteristics to generate a new set of reagents for scientific research. Starting from a synthetic library we plan to validate our strategy selecting antibodies for molecular targets with great demand in the market. In this case, we chose as prototypes beta-actin and human GAPDH proteins, often used as loading controls in western blotting experiments. After validating our pipeline, we will be in position to offer a set of new products including reagents for immunohistochemistry, fluorescence microscopy, chaperones for protein crystallization and other customized services. This proposal has the potential to reduce our country's dependence on this class of high-cost reagents and catalyze innovation in new products and services with high added value. (AU)