| Grant number: | 21/10987-3 |
| Support Opportunities: | Regular Research Grants |
| Start date: | February 01, 2022 |
| End date: | January 31, 2024 |
| Field of knowledge: | Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery |
| Principal Investigator: | José Paes de Oliveira Filho |
| Grantee: | José Paes de Oliveira Filho |
| Host Institution: | Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil |
Abstract
The Equus caballus papillomavirus (EcPV) have been associated with some neoplasia in horses, e.g., classical papillomatosis, genital papilloma and aural plaque. Aural plaque is a cosmopolitan dermatophaty characterized by the presence of circular, whitish lesions up to 3 cm in diameter in the inner surfaces of the pinna and rarely in other regions of the horse's body, regardless of age, breed or gender. The EcPV1, 3, 4, 5, and 6 have previously been associated, alone or in coinfection, with equine aural plaque in Brazil, whereas, EcPV2 and 7 were not identified in these samples. However, due to the recent discovery of EcPV8 and 9, no studies have evaluated the presence of these EcPVs in equine aural plaque samples. Therefore, the aim of this study will be to verify the presence of EcPV8 and 9 DNA by PCR in 102 samples of aural plaque from horses from 30 farms located in different regions of Brazil. Of these samples, 83 were already used in a previous study that evaluated the presence of EcPV1 - 7, while 19 were collected for this study, thus, in these samples, the EcPV1 - 7 will also be evaluated. The specific primers for EcPV8 and 9 will be synthesized and tested in silico, while PCRs for the other EcPVs (1 - 7) will use primers previously described. The PCRs specificities will also be proven by direct Sanger sequencing of positive PCR products. As the association of EcPV8 and 9 to the aural plaque is unknown, mini-genes will be built that will serve as positive PCR controls and, if necessary, mini-genes for the other EcPVs will also be built. Furthermore, the clinical characteristics of the aural plaque will be evaluated according to the EcPV detected. At the end of this experiment, we seek to assess the importance of these viruses in the aetiology of the aural plaque and, if possible, associate them with the clinical alterations of this dermatopathy. (AU)
| Articles published in Agência FAPESP Newsletter about the research grant: |
| More itemsLess items |
| TITULO |
| Articles published in other media outlets ( ): |
| More itemsLess items |
| VEICULO: TITULO (DATA) |
| VEICULO: TITULO (DATA) |