Role of beta 2 adrenoceptor in satellite cell proliferation and differentiation

• Grant number: 20/15351-7
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2022
• End date: August 31, 2024
• Field of knowledge: Health Sciences - Physiotherapy and Occupational Therapy
• Principal Investigator: Elen Haruka Miyabara
• Grantee: Elen Haruka Miyabara
• Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

Satellite cells, a post-natal population of muscle stem cells, are important for post-natal muscle development because they fuse in order to promote the myofiber growth. In addition, satellite cells contribute to the maintenance of muscle mass integrity and are essential for the success of the remarkable regenerative process of skeletal muscle. Overall, skeletal muscle regeneration involves the installation of an inflammatory process, followed by the activation of satellite cells, and their subsequent proliferation. Then, a small fraction of these cells returns to quiescence and most of them differentiates to reconstitute the previously injured muscle tissue. The morphological changes that occur during muscle regeneration are well known, however, the molecular changes involved in this process are not yet fully elucidated. In this context, initial studies suggested that the beta 2 adrenoceptor could be a possible regulator of the muscle regenerative process, by demonstrating that treatment with synthetic beta 2-adrenergic agonists in animals anticipates the proliferation of satellite cells and improves the structure and function of regenerating skeletal muscles. Subsequent works published by our group analyzed the regeneration of skeletal muscles from beta 2-adrenoceptor knockout mice and demonstrated that the beta 2 adrenoceptor is important for the morphological and functional regeneration of skeletal muscle, since this receptor is involved 1) in the regulation of macrophage recruitment to the regenerating muscle tissue, through the activation of TGF- beta receptor I (TbetaR-I) / Smad2 / 3 and reduction in the expression of akirin 1, a macrophage chemoattractant (SILVA et al., 2014); and 2) in the remodeling of intramuscular connective tissue during skeletal muscle regeneration, by reducing the activity of matrix metalloproteinase 9 (MMP-9) (SILVA et al., 2016). Although these studies have reported the importance of the beta 2 adrenoceptor for the muscle regenerative process, the systematic study of the role of the beta 2 adrenoceptor on satellite cell function is still lacking. Therefore, this project aims to investigate the role of the beta 2 adrenoceptor on satellite cell proliferation and differentiation. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DA SILVA, MEIRICRIS TOMAZ; SANTOS, AUDREI R.; KOIKE, TATIANA E.; NASCIMENTO, TABATA L.; ROZANSKI, ANDREI; BOSNAKOVSKI, DARKO; PEREIRA, LYGIA, V; KUMAR, ASHOK; KYBA, MICHAEL; MIYABARA, ELEN H.. The fibrotic niche impairs satellite cell function and muscle regeneration in mouse models of Marfan syndrome. ACTA PHYSIOLOGICA, v. 237, n. 1, p. 19-pg., 2022-10-17. (17/14115-5, 20/15351-7, 18/24946-4, 17/09069-4, 14/23391-8, 13/04783-0, 14/13874-1)

SANTOS, AUDREI R.; KOIKE, TATIANA E.; SANTANA, ALANA M.; MIRANDA, NATALYA C.; DELL AQUILA, RODRIGO A.; SILVA, THIAGO C.; AOKI, MARCELO S.; MIYABARA, ELEN H.. Glutamine supplementation accelerates functional recovery of EDL muscles after injury by modulating the expression of S100 calcium-binding proteins. Histochemistry and Cell Biology, v. 160, n. 2, p. 12-pg., 2023-05-14. (20/15351-7, 17/09069-4, 23/00289-2, 18/24946-4)