Lipid membrane structure via scattering density profiles from chemical groups and membrane remodeling analysis

• Grant number: 22/05434-8
• Support Opportunities: Research Grants - Visiting Researcher Grant - International
• Start date: June 26, 2022
• End date: July 09, 2022
• Field of knowledge: Biological Sciences - Biophysics - Molecular Biophysics
• Principal Investigator: Rosangela Itri
• Grantee: Rosangela Itri
• Visiting researcher: Francesco Spinozzi
• Visiting researcher institution: Università Politecnica delle Marche (UNIVPM), Italy
• Host Institution: Instituto de Física (IF). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The MSDP (Modified Scattering Density Profile) method for the analysis of Small-Angle X-ray Scattering (SAXS) experimental data of self-assembling systems, present in the GENFIT software in planar, cylindrical and spherical geometries, will be integrated by a database of the more common chemical groups constituting lipids and surfactants molecules. A knowledge transfer will be carried out concerning the quantitative analysis, based on a combination of ImageJ, Fortran and Gnuplot scripts, of images obtained with phase-contrast and fluorescence mode optical microscopy on systems formed by Giant Unilamellar Vesicles (GUVs) in the presence of oxidizing agents. (AU)