Biophotonics applied to study of extracellular vesicles as connectors of kidney-heart crosstalk

• Grant number: 22/00153-0
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2022
• End date: July 31, 2024
• Field of knowledge: Biological Sciences - Biophysics - Radiology and Photobiology
• Principal Investigator: Marcela Sorelli Carneiro Ramos
• Grantee: Marcela Sorelli Carneiro Ramos
• Host Institution: Centro de Ciências Naturais e Humanas (CCNH). Universidade Federal do ABC (UFABC). Santo André , SP, Brazil
• Associated researchers: Herculano da Silva Martinho

Abstract

In the current stage of medicine, the clinical diagnosis of a patient is obtained through the analysis of clinical examinations, comparison with standard values of physiological and genetic parameters, high-precision medical images and detection of metabolites in biofluids such as blood or urine. However, even with a broad arsenal available for characterization and measurement of homeostasis, numerous cases of patients with similar symptoms and parameters, but affected by different diseases, are reported. Furthermore, the response of different individuals to the same treatment can be quite different. The establishment of a precise and personalized medical diagnosis and therapy (theranostic) considering the needs and response potential of each individual is one of the great challenges of modern medicine, also called by some precision medicine. Obtaining new biomarkers, characterizing their dynamic molecular response, and developing new therapeutic strategies, considering personalized molecular and genetic aspects, are possible strategies to overcome this challenge. In this project, inflammatory processes of systemic origin, such as cardiorenal syndrome (SCR), will be characterized by vibrational nano-spectroscopy ("Surface Enhancement Raman Spectroscopy" and "Photo-Induced Force Microscopy") as well as by conventional biological techniques and molecular and cellular of the systems involved. Type 3 SCR (SCR3) is characterized by acute kidney injury causing cardiac injury, and has been the subject of laboratory research over the past 10 years. Within the pathophysiology of SCR, extracellular vesicles (EVs) were identified as participating in the communication between kidneys and heart through nucleic acids and proteins. Given the above, this study aims to evaluate the participation of VEs produced by ischemic kidney injury in cardiac changes, using conventional techniques of cell/molecular biology and vibrational nanospectroscopy as a tool for the discovery of possible biomarkers. Thus, the unilateral renal ischemia and reperfusion (I/R) model will be performed for 60 minutes, followed by 8 days of renal reperfusion. Then, the vesicles present in the sera of ischemic and non-ischemic animals will be isolated and characterized using traditional techniques (flow cytometry and SEM), and biophotonics techniques. The main scientific and technological advances that we aim to achieve at the end of the project are: (i) identification of molecular signatures present in EVs after I/R and (ii) identification of new inflammatory biomarkers. (AU)

Scientific publications

(8)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VERNIER, IMARA CARIDAD STABLE; NERES-SANTOS, RAQUEL SILVA; ANDRADE-OLIVEIRA, VINICIUS; CARNEIRO-RAMOS, MARCELA SORELLI. Immune Cells Are Differentially Modulated in the Heart and the Kidney during the Development of Cardiorenal Syndrome 3. CELLS, v. 12, n. 4, p. 17-pg., 2023-02-01. (19/11077-0, 20/02923-2, 22/00153-0)

PIERETTI, JOANA CLAUDIO; FREIRE, BRUNA MOREIRA; ARMENTANO, GIOVANA MARCHINI; SANTANA, BIANCA DE MELO; BATISTA, BRUNO LEMOS; CARNEIRO-RAMOS, MARCELA SORELLI; SEABRA, AMEDEA BAROZZI. Chronic exposure to nitric oxide sensitizes prostate cancer cells and improved ZnO/CisPt NPs cytotoxicity and selectivity. International Journal of Pharmaceutics, v. 640, p. 8-pg., 2023-05-10. (22/00321-0, 20/03646-2, 20/08566-7, 22/00153-0)

STABILE, JEFERSON; NERES-SANTOS, RAQUEL SILVA; HERNANDES, ISABELA DORTA MOLINA; JUNHO, CAROLINA VICTORIA CRUZ; ALVES, GEOVANE FELIPPE; SILVA, ISABELLA CARDOSO; CARNEIRO-RAMOS, MARCELA SORELLI; FURSTENAU, CRISTINA RIBAS. Renal ischemia and reperfusion impact the purinergic signaling in a vascular bed distant from the injured site. Biochimie, v. 222, p. 8-pg., 2024-02-29. (19/12219-3, 22/00153-0, 19/11077-0)

JIMENEZ, GABRIELLE; FOIANI, LETICIA; FIGUEIREDO, JULIA TOLEDO; ALVES, MARIANA SA; RODRIGUES, NAYARA SA; BANDEIRA, CELSO MULLER; ALVES, MONICA GHISLAINE OLIVEIRA; ALMEIDA, JANETE DIAS; MARTINHO, HERCULANO DA SILVA. Histine, carbohydrates/polysaccharides, and proteins with β-sheet conformation as promising staging markers for oral squamous cell carcinoma. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, v. 340, p. 8-pg., 2025-11-05. (19/11077-0, 22/00153-0, 16/08633-0)

CARNEIRO-RAMOS, MARCELA SORELLI; NERES-SANTOS, RAQUEL SILVA; DA SILVA, JESSICA VERONICA; BEDIN, BEATRIZ FAVERO; PARLETTA, ALINE CRISTINA; HONDA, LARA MEI; JUNHO, CAROLINA VICTORIA CRUZ. From AKI to CKD: Role of miRNAs in disease progression. Life Sciences, v. 378, p. 9-pg., 2025-10-01. (23/06583-0, 22/00153-0, 21/12435-8, 23/17588-2)

ARMENTANO, GIOVANA MARCHINI; PIERETTI, JOANA CLAUDIO; FALCONI, CARLOS ALEXANDRE; SEABRA, AMEDEA BAROZZI; CARNEIRO-RAMOS, MARCELA SORELLI. Nitric Oxide Plays a Dual Role in Cardiorenal Syndrome in Vitro Model. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, v. 58, n. 1, p. 16-pg., 2024-01-01. (22/01124-4, 22/00153-0, 19/11077-0, 20/03646-2)

DA SILVA, JESSICA VERONICA; NEPOMUCENO, GABRIELLE TEODORO; BATISTA, ANDRE MOURAO; DA VEIGA, GLAUCIA RAQUEL LUCIANO; FONSECA, FERNANDO LUIZ AFFONSO; CARNEIRO-RAMOS, MARCELA SORELLI; MARTINHO, HERCULANO DA SILVA. Blood collection tube components interference on spectral signatures of chronic kidney disease probed by micro-reflectance Fourier-transform infrared spectroscopy on serum. VIBRATIONAL SPECTROSCOPY, v. 132, p. 10-pg., 2024-03-21. (22/00153-0, 19/11077-0)

SANTANA, BIANCA DE MELO; ARMENTANO, GIOVANA MARCHINI; FERREIRA, DAYANA AGNES SANTOS; DE FREITAS, CAMILA SIMOES; CARNEIRO-RAMOS, MARCELA SORELLI; SEABRA, AMEDEA BAROZZI; CHRISTODOULIDES, MYRON. In Vitro Bactericidal Activity of Biogenic Copper Oxide Nanoparticles for Neisseria gonorrhoeae with Enhanced Compatibility for Human Cells. ACS APPLIED MATERIALS & INTERFACES, v. 16, n. 17, p. 10-pg., 2024-04-18. (21/13821-9, 22/00153-0, 20/08566-7, 22/00321-0)