Polymer (PLA) autogenous bone formation implanted in the iliac bone of sheep as a bone substitute proposal for critical failures

• Grant number: 21/12189-7
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2022
• End date: July 31, 2024
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
• Principal Investigator: Paulo Aléscio Canola
• Grantee: Paulo Aléscio Canola
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated researchers: Carlos Augusto Araújo Valadão ; Luis Gustavo Gosuen Gonçalves Dias

Abstract

Fractures, in general, are amongst the most reported problems in veterinary surgical routine. Despite the large number of existing techniques and materials, fracture resolution still represents a challenge in orthopedics, especially those cases in which non-union, critical size bone defect or multiple fractures are present. In view of these scenarios, there has been a continuous search for the improvement of techniques for the treatment of non-union and the search for materials and techniques that enable better healing of these failures. In this regard, we hereby propose to evaluate the viability of poly lactic acid biopolymer (PDA) implanted in the iliac crest of sheep in order to built-in an autogenous graft for posterior implantation in critical size bone defect sites. For this, 15 mixed breed sheep, weighing between 25 and 40 kg, considered healthy, and with no previous history of injuries related to the musculoskeletal system will be used. The subjects will be submitted to general anesthesia for the surgical implantation of the PDA scaffolds (one per animal) in the ileum. After the implantation of the biopolymer, the animals will be evaluated daily by means of physical examination and weekly by imaging procedures to check the general condition of the animals and the interaction between the implant and the recipient. On the 30th, 45th and 60th days after surgery, seven animals will be euthanized at time and the implants removed and evaluated for osteointegration and osteoinduction properties, by scanning electron microscopy, histopathology, thermography, ultrasonography, radiography and tomographic examination. (AU)