Molecular, cellular and pathophysiological mechanisms on acute renal failure

• Grant number: 04/08311-6
• Support Opportunities: Research Projects - Thematic Grants
• Start date: December 01, 2004
• End date: December 31, 2009
• Field of knowledge: Health Sciences - Medicine - Medical Clinics
• Principal Investigator: Nestor Schor
• Grantee: Nestor Schor
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Pesquisadores principais: Alvaro Pacheco e Silva Filho ; Miguel Cendoroglo Neto
• Associated research grant(s): 09/02492-2 - World Congress of Nephrology 2009, AR.EXT
• Associated scholarship(s): 08/06219-6 - Evaluation of NF-kB intracellular signaling pathway and endostatin on human mesangial cell in endotoxic acute kidney injury during diabetes mellitus, BP.PD; 05/02524-0 - Removal of inflammatory mediators by hemodiafiltration in patients with acute renal failure, BP.PD

Abstract

Despite impressive advances in the field of acute renal failure (ARF), mortality rates (about 50%) are still toa high. Maintenance of this figure in the past 4 decades is a consequence of different factors. Improvement in health care and medical progress allowed severely ill patients to be kept alive long enough to develop ARF. Mas, também, a manutenção desta elevada mortalidade é decorrente do incompleto conhecimento das etapas da cascata fisiopatológica desta doença, desde os fatores desencadeantes intracelulares, passando pelos aspectos de estabelecimento e manutenção até os fatores de correção biológica das alterações induzidas nas células renais.On the other hand, incomplete knowledge of the pathophysiology of ARF, from its origin at the cellular level to development and maintenance of the factors that participate in the resolution renal alterations, are responsible for this unsucefull outcome. Our laboratory has been developing studies in the field for more than 25 years, employing models of toxic or ischemic ARF and different research tools including but not limited to systemic and glomerular heamodynamics analysis, and cell and molecular biology. Thus, this thematic project. aims to study different stages of the pathophysiology of ARF, by means of gene and protein expression analysis, and evaluation of inflammatory and immunologic players in the scenario of toxic or ischemic aggression of renal cells. It also explores new models for ARF induction, as well as experimental protective strategies applicable to clinical practice. In addition, the relevance of experimental findings will be challenged in clinical studies that will concentrate on patients prone to develop ARF. This project was structured to cover different aspects of the pathophysiology of ARF, as a RESEARCH FIELD, with various strategies, aiming a global understanding and with the intention to identify new approaches to interfering with the development and maintenance of this disease. (AU)

Scientific publications

(18)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

M.A. GLORIA; M.A. CENEDEZE; A. PACHECO-SILVA; N.O.S. CÂMARA. The blockade of cyclooxygenases-1 and -2 reduces the effects of hypoxia on endothelial cells. Brazilian Journal of Medical and Biological Research, v. 39, n. 9, p. 1189-1196, 2006-09-00. (04/08311-6)

NOGUEIRA‚ E.; OZAKI‚ K.S.; TOMIYAMA‚ H.; GRANATO‚ C.F.H.; CAMARA‚ N.O.S.; OTHERS. The emergence of cytomegalovirus resistance to ganciclovir therapy in kidney transplant recipients. International Immunopharmacology, v. 6, n. 13, p. 2031-2037, 2006. (04/08311-6)

PONCIANO‚ V.C.; RENESTO‚ P.G.; NOGUEIRA‚ E.; RANGEL‚ É.B.; CENEDEZE‚ M.A.; FRANCO‚ M.F.; CÂMARA‚ N.O.S.; PACHECO-SILVA‚ A.. Tim-3 expression in human kidney allografts. TRANSPLANT IMMUNOLOGY, v. 17, n. 3, p. 215-222, 2007. (04/08311-6)

NOGUEIRA‚ E.; PONCIANO‚ V.C.; NAKA‚ É.L.; MARQUES‚ G.D.M.; CENEDEZE‚ M.A.; C{\=A}MARA‚ N.O.S.; PACHECO-SILVA‚ A.. Toll-like receptors-related genes in kidney transplant patients with chronic allograft nephropathy and acute rejection. International Immunopharmacology, v. 9, n. 6, p. 673-676, 2009. (04/08311-6)

WANG‚ P.H.M.; CENEDEZE‚ M.A.; CAMPANHOLLE‚ G.; MALHEIROS‚ D.M.A.C.; DE MOURA TORRES‚ H.A.; PESQUERO‚ J.B.; PACHECO-SILVA‚ A.; C{\=A}MARA‚ N.O.S.. Deletion of bradykinin B1 receptor reduces renal fibrosis. International Immunopharmacology, v. 9, n. 6, p. 653-657, 2009. (04/08311-6, 06/03982-5, 07/07139-3)

PEREIRA‚ M.G.; CÂMARA‚ N.O.S.; CAMPAHOLLE‚ G.; CENEDEZE‚ M.A.; DE PAULA ANTUNES TEIXEIRA‚ V.; DOS REIS‚ M.A.; PACHECO-SILVA‚ A.. Pioglitazone limits cyclosporine nephrotoxicity in rats. International Immunopharmacology, v. 6, n. 13, p. 1943-1951, 2006. (04/08311-6)

FERNANDES BERTOCCHI, ANA PAULA; CAMPANHOLE, GABRIELA; MEI WANG, PAMELLA HUEY; GONCALVES, GISELLE MARTINS; DAMIAO, MARCIO JOSE; CENEDEZE, MARCOS ANTONIO; BERALDO, FELIPE CAETANO; ANTUNES TEIXEIRA, VICENTE DE PAULA; DOS REIS, MARLENE ANTONIA; MAZZALI, MARILDA; et al. A Role for galectin-3 in renal tissue damage triggered by ischemia and reperfusion injury. Transplant International, v. 21, n. 10, p. 999-1007, OCT 2008. (04/08311-6)

MACIEL, THIAGO T.; COUTINHO, ENIA L.; SOARES, DEBORA; ACHAR, EDUARDO; SCHOR, NESTOR; BELLINI, MARIA H.. Endostatin, an antiangiogenic protein, is expressed in the unilateral ureteral obstruction mice model. JOURNAL OF NEPHROLOGY, v. 21, n. 5, p. 753-760, SEP-OCT 2008. (04/08311-6)

MEI WANG, PAMELLA HUEY; SCHWINDT, TELMA T.; BARNABE, GABRIELA FILOSO; MOTTA, FABIANA L. T.; SEMEDO, PATRICIA; BERALDO, FELIPE CAETANO; MAZZALI, MARILDA; DOS REIS, MARLENE ANTONIA; ANTUNES TEIXEIRA, VICENTE DE PAULA; PACHECO-SILVA, ALVARO; et al. Administration of Neural Precursor Cells Ameliorates Renal Ischemia-Reperfusion Injury. NEPHRON EXPERIMENTAL NEPHROLOGY, v. 112, n. 1, p. E20-E28, 2009. (07/07139-3, 04/08311-6)

CHRISTO, JOELMA SANTINA; RODRIGUES, ADELSON MARCAL; MOURO, MARGARET GORI; CENEDEZE, MARCOS ANTONIO; SIMOES, MANUEL DE JESUS; SCHOR, NESTOR; SUEMITSU HIGA, ELISA MIEKO. Nitric oxide (NO) is associated with gentamicin (GENTA) nephrotoxicity and the renal function recovery after suspension of GENTA treatment in rats. NITRIC OXIDE-BIOLOGY AND CHEMISTRY, v. 24, n. 2, p. 77-83, MAR 15 2011. (04/08311-6)

H.S. PINHEIRO; N.O.S. CAMARA; I.L. NORONHA; I.L. MAUGERI; M.F. FRANCO; J.O.A.P. MEDINA; A. PACHECO-SILVA. Contribution of CD4+ T cells to the early mechanisms of ischemia- reperfusion injury in a mouse model of acute renal failure. Brazilian Journal of Medical and Biological Research, v. 40, n. 4, p. 557-568, 2007-04-00. (04/08311-6)

R. CHINEN; N.O.S. CÂMARA; S. NISHIDA; M.S. SILVA; D.A. RODRIGUES; A.B. PEREIRA; A. PACHECO-SILVA. Determination of renal function in long-term heart transplant patients by measurement of urinary retinol-binding protein levels. Brazilian Journal of Medical and Biological Research, v. 39, n. 10, p. 1305-1313, 2006-10-00. (04/08311-6)

PONCIANO‚ V.C.; SOARES‚ M.F.; NAKA‚ É.L.; ARRUDA‚ É.F.; CENEDEZE‚ M.A.; FRANCO‚ M.F.; PACHECO-SILVA‚ A.; C{\=A}MARA‚ N.O.S.. Urinary CD20 mRNA as a surrogate of CD20-positive cells infiltration during allograft dysfunction in renal transplant patients. International Immunopharmacology, v. 9, n. 6, p. 663-667, 2009. (04/08311-6)

WANG‚ P.H.M.; CENEDEZE‚ M.A.; PESQUERO‚ J.B.; PACHECO-SILVA‚ A.; CÂMARA‚ N.O.S.. Influence of bradykinin B1 and B2 receptors in the immune response triggered by renal ischemia-reperfusion injury. International Immunopharmacology, v. 6, n. 13, p. 1960-1965, 2006. (04/08311-6)

NAKA‚ E.L.; PONCIANO‚ V.C.; CENEDEZE‚ M.A.; PACHECO-SILVA‚ A.; SARAIVA CÂMARA‚ N.O.. Detection of the Tim-3 ligand‚ galectin-9‚ inside the allograft during a rejection episode. International Immunopharmacology, v. 9, n. 6, p. 658-662, 2009. (04/08311-6, 07/07139-3)

GONÇALVES‚ G.M.; CENEDEZE‚ M.A.; FEITOZA‚ C.Q.; BATISTA DE PAULA‚ C.; MACUSSO‚ G.D.; PINHEIRO‚ H.S.; TEIXEIRA‚ V.P.A.; DOS REIS‚ M.A.; PACHECO-SILVA‚ A.; CÂMARA‚ N.O.S.. Heme oxygenase 1 and renal ischemia and reperfusion injury: The impact of immunosuppressive drug. International Immunopharmacology, v. 6, n. 13, p. 1966-1972, 2006. (04/08311-6)

WANG, PAMELLA H. M.; CAMPANHOLLE, GABRIELA; CENEDEZE, MARCOS A.; FEITOZA, CARLA Q.; GONCALVES, GISELLE M.; LANDGRAF, RICHARDT G.; JANCAR, SONIA; PESQUERO, JOAO B.; PACHECO-SILVA, ALVARO; CAMARA, NIELS O. S.. Brabykinin B1 Receptor Antagonism Is Beneficial in Renal Ischemia-Reperfusion Injury. PLoS One, v. 3, n. 8, p. e3050, 2008. (07/07139-3, 06/03982-5, 04/08311-6)

MARQUES‚ V.P.; GONÇALVES‚ G.M.; FEITOZA‚ C.Q.; CENEDEZE‚ M.A.; FERNANDES BERTOCCHI‚ A.P.; DAMIAO‚ M.J.; PINHEIRO‚ H.S.; ANTUNES TEIXEIRA‚ V.P.; DOS REIS‚ M.A.; PACHECO-SILVA‚ A.; et al. Influence of TH1/TH2 switched immune response on renal ischemia-reperfusion injury. NEPHRON EXPERIMENTAL NEPHROLOGY, v. 104, n. 1, p. e48-e56, 2006. (04/08311-6)