Characterization of the inflammatory response in an experimental model of Extraparenchymal Neurocysticercosis

• Grant number: 22/03042-5
• Support Opportunities: Research Grants - Initial Project
• Start date: February 01, 2023
• End date: January 31, 2028
• Field of knowledge: Health Sciences - Medicine - Surgery
• Principal Investigator: Pedro Tadao Hamamoto Filho
• Grantee: Pedro Tadao Hamamoto Filho
• Host Institution: Faculdade de Medicina (FMB). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
• Associated researchers: Adriana Camargo Ferrasi ; Agnès Fleury ; Alexandre Todorovic Fabro ; Edda Lydia Sciutto Conde ; Estela de Oliveira Lima ; Marco Antônio Zanini ; Vânia Maria de Vasconcelos Machado
• Associated scholarship(s): 24/19907-0 - Morphological Analysis of Sexual Dimorphism in an Experimental Model of Extraparenchymal Neurocysticercosis-Induced Hydrocephalus in Rats, BP.IC; 23/12860-6 - Identification of inflammatory cytokines recruited in experimental extraparenchymal neurocysticercosis at different stages of evolution., BP.MS

Abstract

Neurocysticercosis is the most common parasitic disease of the central nervous system. It occurs by the cerebral infestation by the larva of Taenia solium, which happens when eggs of the parasite are ingested. The prevalence of the disease decreased at the end of the 20th century due to sanitary measures, but it remains endemic in several regions of Latin America, sub-Saharan Africa and Southeast Asia. Migratory flows have determined the emergence of new cases in the United States and Europe. Experimental models of neurocysticercosis have been developed in the last decade, with a special focus on the parenchymal form of the disease. On the other hand, the extraparenchymal form of neurocysticercosis is more aggressive and raises more controversies, especially regarding therapeutic management, with lower levels of evidence for treatment recommendations. Therefore, we developed a model of extraparenchymal neurocysticercosis in rats by subarachnoid inoculation of Taenia crassiceps cysts. This model reproduces several radiological and morphological aspects of human neurocysticercosis and opens perspectives for laboratory investigation of the disease. In this project, we intend to investigate whether there is a change in the inflammatory pattern in extraparenchymal neurocysticercosis at different times of infection; whether sex is a determinant of morphological and inflammatory differences in experimental extraparenchymal neurocysticercosis; and to investigate the role of immunomodulatory drugs in the response to antiparasitic treatment. For this, we will employ histomorphometric analysis, enzyme immunoassay analysis for quantification of inflammatory cytokines and analysis of expression of genes involved in the inflammatory response through real-time polymerase chain reaction. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OLIVEIRA, VINICIUS TADEU; MARTINS, TATIANE DE CAMARGO; CONCEICAO, RENATO TAVARES; GENEROSO, DIEGO; MACHADO, VANIA MARIA DE VASCONCELOS; BATAH, SABRINA SETEMBRE; FABRO, ALEXANDRE TODOROVIC; ZANINI, MARCO ANTONIO; SCIUTTO, EDDA; FLEURY, AGNES; et al. Murine Extraparenchymal Neurocysticercosis: Appropriate Model for Evaluating Anthelminthic and Anti-Inflammatory Treatment Schedules. TROPICAL MEDICINE AND INFECTIOUS DISEASE, v. 9, n. 9, p. 7-pg., 2024-09-01. (22/03042-5)

GENEROSO, DIEGO; MARTINS, TATIANE DE CAMARGO; CAMACHO, CAMILA RENATA CORREA; SEGREDO, MANUELLA PACIFICO DE FREITAS; BATAH, SABRINA SETEMBRE; FABRO, ALEXANDRE TODOROVIC; SCIUTTO, EDDA; FLEURY, AGNES; HAMAMOTO FILHO, PEDRO TADAO; ZANINI, MARCO ANTONIO. Oxidative Stress in the Murine Model of Extraparenchymal Neurocysticercosis. MICROORGANISMS, v. 12, n. 9, p. 9-pg., 2024-09-01. (22/03042-5)