Development of a versatile 3D scanning unit for automated combined time-resolved detection of various characteristic emissions in the Vis-NIR

• Grant number: 23/06807-5
• Support Opportunities: Research Grants - Visiting Researcher Grant - International
• Start date: March 17, 2024
• End date: May 01, 2024
• Field of knowledge: Biological Sciences - Biophysics - Radiology and Photobiology
• Principal Investigator: Mauricio da Silva Baptista
• Grantee: Mauricio da Silva Baptista
• Visiting researcher: Steffen Hackbarth
• Visiting researcher institution: Humboldt University, Germany
• Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
• Associated research grant: 13/07937-8 - Redoxome - Redox Processes in Biomedicine, AP.CEPID

Abstract

Singlet oxygen 1Deltag (1O2) is the first excited state of molecular oxygen with 94.3 kJ/mol in excess to its ground triplet state, and with a chemical reactivity completely different from that of molecular oxygen, as it can attack chemical bonds by two electron oxidations. 1O2 plays important roles in materials science, physics, chemistry, biology and medicine, being primarily responsible for photosensitized oxidations. It has also been postulated that 1O2 participates in the activation of neutrophils, in the mechanism of sepsis and in the redox signaling networks of various cells. However, knowledge of the real functions of 1O2 has been hampered by the difficulty in obtaining its image profiles that could verify its presence and reactivity in different environments. In recent years, great improvements have been achieved in real-time tracking of 1O2 by different detection methods, including detection of the emission characteristic around 1270 nm, detection of delayed photosensitizer fluorescence, detection of photosensitizer phosphorescence. The objective of this proposal is to build a spectrophosphorometer with 3D functionality to allow the acquisition of spatial resolution scans of emission signals with time-resolved detection, in the wavelength range of 400-1400 nm (using appropriate filters and optics). We ask FAPESP to support the visit of Prof. Steffen Hackbarth, who will build the equipment and who will participate in several academic activities, including being speaker at two international congresses (SBBf and Elafot-LatASP). Most of the parts (and the most expensive ones) needed to build the equipment are already available in the laboratory of prof. Baptista. The acquisition of new photonic parts will be financed by the CEPID-Redoxoma project. (AU)