EVALUATION OF PHOTODYNAMIC THERAPY WITH DIFFERENT COMPOUNDS FOR MICROBIAL INACTIVATION - IN VITRO AND IN VIVO ASSAYS

• Grant number: 22/10251-0
• Support Opportunities: Regular Research Grants
• Start date: November 01, 2023
• End date: October 31, 2025
• Field of knowledge: Biological Sciences - Biophysics - Radiology and Photobiology
• Principal Investigator: Juliana Guerra Pinto
• Grantee: Juliana Guerra Pinto
• Host Institution: Instituto de Pesquisa e Desenvolvimento (IP&D). Universidade do Vale do Paraíba (UNIVAP). São José dos Campos , SP, Brazil
• Associated researchers: ALEJANDRO GUILLERMO MIÑAN ; Andreza Ribeiro Simioni ; Isabelle Ferreira ; Juliana Ferreira Strixino ; Kumiko Koibuchi Sakane ; Martha Simões Ribeiro ; SALVATORE SORTINO

Abstract

Bacteria are important in several biological processes, as well as used in industrial and physiological processes. The human skin is the largest organ in our body, responsible for the protection of the internal environment and the interaction of the organism with the environment. Bacteria are found in large quantities in our gastrointestinal tract and oral cavity, for example, in addition to colonizing our skin, constituting the human microbiota. The microbiota plays a crucial role in the protection that our skin provides to the body, helping to maintain skin acidity and preventing colonization by pathogenic microorganisms. However, the ability of bacteria to develop resistance to antibiotics becomes the biggest threat to human health in the 21st century, with estimates of 10 million deaths from resistance by 2050. Infections with resistant bacteria increase a patient's hospital stay, increase the expenses and increase the risk of death. The need for alternative therapies capable of eliminating microorganisms without creating resistance is a reality. PDT has shown promising results in the treatment of various lesions and bacterial inactivation. The present project aims to evaluate the cytotoxicity of curcumin encapsulated in anionic and cationic bovine serum albumin (BSA) nanoparticles and Nitric Oxide Photodonor Rhodamine in clinical strains of Staphylococcus aureus and Pseudomonas aeruginosa, in vitro, and to evaluate the effect of PDT with different formulations of nanoencapsulated curcumin and Nitric Oxide Photo Donor compound in lesions infected with S. aureus and P. aeruginosa in vivo. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PINTO, JULIANA GUERRA; LANERI, FRANCESCA; DE PAULA RIBEIRO BRAMBILLA, ISABELLE; DE AZEVEDO, MARIA CLARA BARBOSA SILVA; AMBROSIO, JESSICA A. R.; SIMIONI, ANDREZA R.; FRAIX, AURORE; SORTINO, SALVATORE; FERREIRA-STRIXINO, JULIANA. Curcumin-loaded bovine serum albumin (BSA) nanoparticles: photoreactivity and photodynamic action against Acinetobacter baumannii and Staphylococcus aureus. NEW JOURNAL OF CHEMISTRY, v. 48, n. 31, p. 11-pg., 2024-07-02. (22/10251-0)

MARTINS, TASSIA J.; PARISI, CRISTINA; PINTO, JULIANA GUERRA; BRAMBILLA, ISABELLE DE PAULA RIBEIRO; MELILLI, BARBARA; ALEO, DANILO; FERREIRA-STRIXINO, JULIANA; SORTINO, SALVATORE. Simultaneous photoactivation of a fluoroquinolone antibiotic and nitric oxide with fluorescence reporting. JOURNAL OF MATERIALS CHEMISTRY B, v. 12, n. 31, p. 9-pg., 2024-07-09. (22/10251-0)