Advanced search
Start date
Betweenand

Histone H3/H4 acetylation and gene regulation analysis applied to malignancy criteria and tumor aggressiveness in parathyroid carcinomas

Grant number: 23/11261-1
Support Opportunities:Regular Research Grants
Start date: October 01, 2024
End date: September 30, 2026
Field of knowledge:Health Sciences - Medicine - Surgery
Principal Investigator:Luiz Carlos Conti de Freitas
Grantee:Luiz Carlos Conti de Freitas
Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated researchers: Fernando Chahud ; Luciana Oliveira de Almeida ; Rogerio Moraes Castilho

Abstract

Parathyroid carcinoma is manifested by a marked increase in serum levels of calcium and parathyroid hormone, representing 1% to 5% of patients with primary hyperparathyroidism. Its histopathological characteristics frequently do not allow a precise differentiation between benign diseases such as parathyroid adenoma and carcinomas. Furthermore, the molecular mechanisms involved in the development of carcinoma are not completely understood. Epigenetic mechanisms have been studied with the objective of identifying characteristics that differentiate neoplasms and clarify pathophysiological mechanisms, among which, histone acetylation. The imbalance between acetylation and deacetylation of histones seems to favor the development of tumors. This study aims to demonstrate the histone acetylation profile in parathyroid carcinomas. Fifteen parathyroids of patients diagnosed with carcinoma who underwent parathyroidectomy between 1990 and 2022 at the Head and Neck Surgery Service at the USP campus in Ribeirão Preto will be analyzed, in addition to 15 adenomas and 15 samples of normal tissue. Clinical, demographic and laboratory data will be collected from the included patients and the degree of histone acetylation in parathyroid tissues removed by immunofluorescence will be determined, as well as the expression of genes that encode enzymes that regulate histone acetylation, by quantitative PCR. Additionally, the expression of markers related to tumor aggressiveness, such as cancer stem cell (SOX2), cell proliferation (Ki-67) and CDC73/HRPT2 mutation (parafibromin), as well as serum PTH and calcium measurements are analyzed. Data will be analyzed to determine correlations between histone findings and histopathological diagnosis with markers of tumor aggressiveness. (AU)

Articles published in Agência FAPESP Newsletter about the research grant:
More itemsLess items
Articles published in other media outlets ( ):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)