COMBINED TRAINING ANSWERS ON THE EXPRESSION OF CIRCULATING MICRORNAS IN EUTROPHIC, OBESE AND WITH TYPE 2 DIABETES INDIVIDUALS

• Grant number: 24/13126-7
• Support Opportunities: Regular Research Grants
• Start date: March 01, 2025
• End date: February 29, 2028
• Field of knowledge: Health Sciences - Physical Education
• Principal Investigator: Claudia Regina Cavaglieri
• Grantee: Claudia Regina Cavaglieri
• Host Institution: Faculdade de Educação Física (FEF). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Guilherme Defante Telles ; Mara Patricia Traina Chacon Mikahil ; Marcelo Alves da Silva Mori ; Renata Garbellini Duft

Abstract

This research project will continue and complement the FAPESP projects 2016/08751-3 and 2017/11033-8 and will have the following objectives: 1° To evaluate the acute responses of aerobic exercise (AE), strength exercise SE) and combined exercise (CE), in addition of combined training (CT) responses on the global expression of miRNAs in circulating extracellular vesicles from a pool of samples from eutrophic, obese and type 2 diabetes and overweight individuals (T2DMO), in middle age, and still establish which which miRNAs are relevant among the three populations and in responding to chronic training and exercise sessions; 2° Validate the results obtained in the pool sample by evaluating the chronic CT responses on the expression of relevant miRNAs in extracellular vesicles from individual samples from each obese and DM2EP volunteers, in addition to associating these results with clinical cardiometabolic markers. The sample is composed of eutrophic, obese and T2DMO individuals, of both sexes, age of 40 to 60 years and body mass index (BMI) 18.5 to 35kg/m² (varying depending on the population), distributed in a control group with T2DMO (CGT2DM), obese control group (CGO), eutrophic control group (CGE), combined training group with T2DMO (CTGT2DM), obese combined training group (CTGO) and eutrophic combined training group (CTGE). The project has already been approved by the ethics committee. Both groups underwent different assessments (body composition, thermogenic, dietary recall) and had fasting blood collected (all blood samples are stored in the biorepository in a -80°C freezer). Participants subsequently underwent two weeks of familiarization and functional assessments (strength and aerobic capacity), and then the CTGT2DM, CTGE and CTGO groups participated in the acute CT, AT and ST sessions (randomized order by draw and in a cross over design). Before and immediately after each of these sessions, participants underwent new blood collections. After these initial sessions, there was a 16-week training period for CTGT2DM, CTGE and CTGO and/or control condition for CGT2DM, CGE and CGO. At the end of the 16-week period of training and control condition, participants from the six groups underwent initial assessments again, with only the CTGT2DM, CTGE and CTGO undergoing a protocol similar to the first CT session, but with adjusted training loads. The CT program consisted of ST (~35 min, 1-3 sets of 8 to 12 repetitions, 50 to 75% of 1RM, 1 to 1 min and 30 sec interval) followed by AT (35 min. at 50-60% of VO2max) in the same session, performed 3 times a week, for 16 weeks. The acute sessions followed an intensity close to the chronic CT, but with reduced volume and equalized between sessions. In all groups and moments of blood collection, sequencing of miRNAs will now be carried out in extracellular vesicles from the volunteers' sample pool and, using bioinformatics, it will be established which miRNAs are most relevant in differentiating populations and the effects of training/sessions. exercises. After establishing the relevant miRNAs, there will be validation of these targets in pre and post extracellular vesicles samples from each individual in the chronic experimental period, each subject from CTGT2DM, CTGO, CGT2DM and CGO, in addition to the pre-CTGE period. It is expected that different patterns of expression of miRNAs will be found between populations and on the acute effects of the three types of exercise and chronic CT, which will be associated with the improvement of already analysed cardiometabolic markers. (AU)