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EMU: Upgrade on the transmission electron microscope Talos Arctica

Abstract

Cryogenic electron microscopy (cryoEM) has undergone a revolution over the last years, allowing us to obtain high-resolution protein structures using minimal amounts of sample. The evolution that cryoEM experienced in recent years has been marked by several developments including microscopes, detectors, energy filters, sample preparation, as well as in data processing. However, acquiring sufficient data to reconstruct the three-dimensional structure of a biological macromolecule is still commonly a challenge in cryoEM. Among recent advances, it is worth highlighting that several of them aim to establish high-throughput cryo-EM, aiming to reduce the time required for collections. LNNano/CNPEM offers a complete infrastructure in cryoEM, thus playing a fundamental role in establishing the technique in Brazil and the state of São Paulo. LNNano currently hosts four cryo microscopes, currently attending dozens of proposals per year. In particular, the Talos Arctica cryo-microscope, operational since 2018, has been widely used by users to assess sample quality and collect high-resolution data. Given the large number of proposals received and attended by LNNano, investments are needed to reduce data collection time, enabling access to more users and proposals. The items requested in this EMU project consist of a series of updates and repairs, to be implemented in the Talos Arctica cryogenic electron microscope, aiming to increase the facility's productivity and data quality. (AU)

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