Aquisition of Liminex 200 for high throughput siglnaing titration

• Grant number: 23/15845-8
• Support Opportunities: Research Infrastructure Program - Small Medium Sized
• Start date: September 01, 2024
• End date: August 31, 2027
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Jörg Kobarg
• Grantee: Jörg Kobarg
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated researchers: Catarina Raposo Dias Carneiro ; Gislaine Ricci Leonardi ; Karina Cogo Müller

Abstract

The presente proposal envisions the aquisition of a Luminex 200 equipment as a platform to identify and Analyse up to 100 analites (proteins in biological samples) simultâneos per well, in substitution for traditional western blot techniques. The platform works with custom made or comercial kits, that allow a complete investigation of diverse molecular contexts like signaling pathways, imunological resposes, virolgy, câncer, metabolismo, neursciences, toxicity among many other applications. Furhtermore biological sample include urine, blood, cell lysates , culture supernatants and many others. In Campinas there are currently only 3 heavly overdemanded equipmanets of this kind. This kind of equipment is used by many international competitive groups since it allows massive parallel data aquisition, allowing high impact , cutting edge publications. (AU)