EMU: Acquisition of a Confocal Microscope with white light laser and high-sensitivity photonic detectors for "space" biology: in vivo and in vitro evaluation of cell dynamics.

• Grant number: 23/16936-7
• Support Opportunities: Research Infrastructure Program - Small Medium Sized
• Start date: September 01, 2024
• End date: August 31, 2027
• Field of knowledge: Biological Sciences - Parasitology - Protozoology of Parasites
• Principal Investigator: Sergio Schenkman
• Grantee: Sergio Schenkman
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated researchers: Alexandre de Castro Keller ; Ana Claudia Trocoli Torrecilhas ; Ana Flavia Popi ; Carla Claser ; Daniela Santoro Rosa ; Jose Ronnie Carvalho de Vasconcelos ; Karina Possa Abrahão ; Nilmar Silvio Moretti

Abstract

Spatial biology encompasses a variey of technological techniques that are used to investigate the influence of the microenvironment on cellular dynamics in different contexts. One of these approaches is the laser microscopy, which enables the simultaneous analysis of several proteins in fixed material or in living cells, enabling in situ observation of cellular dynamics at the molecular level. This technique has experienced significant advances in recent years, continually updating itself and expanding the potential for analysis at cellular and tissue levels. In this context, we requested the acquisition of a 3D microscopy system, notably the STELLARIS 5, with automated excitation/emission and spectral acquisition capabilities on a nanometric scale. This equipment will be integrated into a cell incubation system already existing in our unit. Furthermore, the proposed system will make it possible to capture images in real time and analyze the lifetime of fluorochromes through the innovative Tausense system, which is still unknown in Brazil. This functionality will provide information on temporal dimensions (4D), as well as on emission/excitation energy variations (5D), allowing detailed analysis of intra- and intercellular interactions. It is noteworthy that the proposed platform can be used with in vivo models, an option currently unavailable in the University's existing systems. Furthermore, the acquisition of the STELLARIS 5 aims to replace the current confocal microscope in operation for 23 years, which presents intermitent errors and lacks spare parts. The technological increase provided by this new equipment will result in more reproducible experiments, better observation of samples, and, in general, a higher level of praticality. In this way, the proposed investment will play a crucial role in scientific advancement not only at Unifesp, but also in other institutions throughout the country. (AU)