Complete dentures obtained by additive manufacturing compared to conventional manufacturing - Crossover, randomized and controlled study

• Grant number: 24/13882-6
• Support Opportunities: Regular Research Grants
• Start date: June 01, 2025
• End date: May 31, 2028
• Field of knowledge: Health Sciences - Dentistry - Dental Clinics
• Principal Investigator: Cláudia Helena Lovato da Silva
• Grantee: Cláudia Helena Lovato da Silva
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: Adriana Barbosa Ribeiro ; Ana Paula Macedo ; Evandro Watanabe
• Associated scholarship(s): 25/10451-7 - Evaluation of Hygiene Protocols Applied to Complete Dentures Obtained by Additive Manufacturing Compared to Conventional Manufacturing - Crossover, Randomized and Controlled Clinical Study, BP.DR

Abstract

This crossover, randomized and controlled clinical study aims to compare complete dentures obtained by additive manufacturing with complete dentures obtained by conventional method using patient-centered variables and the biological and physical behavior of the materials. Furthermore, the antibiofilm effectiveness of hygiene protocols will be compared depending on the type of material used to make the prostheses. Completely edentulous individuals (n=26) who meet the eligibility criteria will use complete dentures made using additive manufacturing (PT3D) and made using the conventional method (PTC), in a randomized and cross-over form. During the experimental period, participants will be instructed to use hygiene protocols for 14 days [brushing and immersion in 0.25% sodium hypochlorite (EHS) and brushing and immersion in 0.15% triclosan (ET)]. The protocols will be instituted in a randomized and cross-over form with a 7-day washout period. Response variables will be measured at Baseline (quality of life related to oral health, satisfaction and quality of prostheses; microbial load of the prosthesis in use and measurement of the color of the prosthesis to be installed), after 2 months of installation of the prostheses (quality of life related to oral health, satisfaction and quality of prostheses; microbial load through culture and counting of colony-forming units and by the FISH method - fluorescence in situ hybridization) and after the use of hygiene protocols to analyze antibiofilm efficacy (biofilm removal by area, identification and microbial load by culture and colony-forming units, cellular metabolism, formation of Candida spp. Pseudohyphae/ hyphae).The data will be evaluated for normality (Shapiro-Wilk test) and homoscedasticity (Levene test). definition of the statistical tests to be used, considering a 95% confidence interval (SPSS 21.0 software). (AU)