Bioactivity of pulp regeneration materials on cellular response and protein synthesis in stem cells from human exfoliated deciduous teeth.

• Grant number: 24/16746-6
• Support Opportunities: Regular Research Grants
• Start date: August 01, 2025
• End date: July 31, 2028
• Field of knowledge: Health Sciences - Dentistry - Pediatric Dentistry
• Principal Investigator: Thais Marchini de Oliveira Valarelli
• Grantee: Thais Marchini de Oliveira Valarelli
• Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
• Associated researchers: Rodrigo Cardoso de Oliveira

Abstract

The purpose of this study will be to analyze the cellular response and protein synthesis by different types of human deciduous dental pulp stem cells in vital and non-vital pulp therapy materials. The samples will be obtained by primary culture of human deciduous tooth stem cells from two tissue sources, the dental pulp and the apical papilla. There are no reports in the literature on the possible morphological and genotypic differences between stem cells from the pulp of exfoliated human deciduous teeth (SHED) and stem cells from the dental papilla of exfoliated human deciduous teeth (SCAPd). Characterization will be done by flow cytometry according to the following markers: Positive (CD105, CD73, CD90, CD44 and CD49f) and negative (CD11b, CD19, CD34, CD45, HLA-DR). In addition, the nature of Human Mesenchymal Stem Cells (MSCs) will be proven through osteogenic, chondrogenic and adipogenic differentiation. The following experimental groups will be evaluated: BBio Membrane, Biodentine, Cimmo HD®, Hydropast®, FeaPex® paste and Guedes-Pinto® paste, on 2 cell types: SHED and SCAPd. Experiments will be carried out on cell viability, cell migration, immunofluorescence, gene expression and protein synthesis for odontogenic, osteogenic and angiogenic markers using the RT-PCR method and the enzyme-linked immunosorbent assay, respectively. The Shapiro-Wilk normality test will be applied in the statistical analysis, as these are quantitative measurements. For data with a normal distribution, intra-group and inter-group comparisons will be made using the repeated measures analysis of variance test, followed by the Tukey test. For non-normally distributed data, intra-group comparisons will be made using the Friedman test and inter-group comparisons using the Kruskal-Wallis test. Pearson's Correlation and Multivariate Linear Regression Analysis will be applied to evaluate the results found. For all tests, the significance level adopted will be 5%. (AU)