EVALUATION OF INTRAOSSEOUS VERSUS INTRA-ARTICULAR MORPHINE IN LPS-INDUCED LAMENESS IN CHIKENS

• Grant number: 24/17558-9
• Support Opportunities: Regular Research Grants
• Start date: September 01, 2025
• End date: August 31, 2027
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Principal Investigator: Caio José Xavier Abimussi
• Grantee: Caio José Xavier Abimussi
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Associated researchers: André Escobar ; Bruno Watanabe Minto ; Carlos Augusto Araújo Valadão ; Guilherme Augusto Marietto Gonçalves

Abstract

Pain can modify a bird's social interactions, such as roosting away from the group, reduced self-cleaning and social interaction with the guardian or keeper. Recognizing and assessing of pain in birds is more difficult. In addition to the limited data, there are also variations in individual behavior between species at the time of interaction with the evaluator. This study aims to evaluate and compare the efficacy of intraosseous and intra-articular morphine injections in inflammatory joint pain, assessing probable changes in walking on the Strideway Gait Analysis System - High Resolution pressure-sensitive walkway. A total of 68 animals will be used. Preliminarily, to determine the dose of LPS that induces joint inflammation, three groups of 12 birds will be formed, which will receive LPS at a dose of 1 µg.kg-1, 3 µg.kg-1 and 7 µg.kg-1, via the intra-articular route. Once the standard LPS dose has been determined, another 56 birds will be divided into 4 groups to induce the joint inflammatory reaction. Morphine will then be applied randomly intra-articularly (0.05 mg.kg-1) or intraosseously (2 mg.kg-1) ipsilateral or contralateral to the affected joint. Subsequently, the animals will be returned to the gait analysis walkway so that comparative post-treatment parameters can be constructed. The data will be submitted to the Shapiro-Wilk normality test and the parametric variables will be submitted to analysis of variance (ANOVA) with repeated measures followed by Tukey's test and presented as means ± standard deviation. Non-parametric variables will be subjected to the Friedman test with Dunn's post-test. Differences will be considered significant when p < 0.05. (AU)