Avian infectious bronchitis: interactions between genetic and antigenic variability among infectious bronchitis virus strains and consequences on immunity, immunodiagnosis and immunoprophylaxis of this diasease

• Grant number: 01/14950-3
• Support Opportunities: Research Projects - Thematic Grants
• Duration: July 01, 2002 - September 30, 2007
• Field of knowledge: Agronomical Sciences - Veterinary Medicine
• Principal Investigator: Helio Jose Montassier
• Grantee: Helio Jose Montassier
• Host Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
• Pesquisadores principais: Leonardo José Richtzenhain

Abstract

The aim of this research is to address the scientific and technical development required forthe application of modern and efficacious techniques which are directly linked to thevirology, immunology and molecular biology, in order to produce the essential reagents, as recombinant antigens of infectious bronchitis virus (IBV), phage display chicken monoclonal antibodies, primers and oligo-probes, so we can standardise and runserological assays, particularly the immunoenzymatic reactions (ELISA), with conventionalor recombinat antigens and poli or monoclonal antibodies, and the molecular biological techniques, as RI -PCR, RT-Nested-PCR, ELOSA and Cycle Sequecing to perform more accurately the diagnosis and consequently the detection or identification of the genetic and the antigenic variants of the IBV or the correspondent immune responses against this virus in vaccinated chickens, as well as to evaluate alternative immunogen preparations, like the IBV recombinant antigens raised in yeast. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

OKINO, CINTIA HIROMI; SILVA MONTASSIER, MARIA DE FATIMA; DE OLIVEIRA, ANDRESSA PERES; MONTASSIER, HELIO JOSE. Rapid detection and differentiation of avian infectious bronchitis virus: an application of Mass genotype by melting temperature analysis in RT-qPCR using SYBR Green I. JOURNAL OF VETERINARY MEDICAL SCIENCE, v. 80, n. 4, p. 725-730, APR 2018. (01/14950-3)

DOS SANTOS, ROMEU MOREIRA; FERNANDO, FILIPE SANTOS; SILVA MONTASSIER, MARIA DE FATIMA; SILVA, KETHERSON RODRIGUES; LOPES, PRISCILA DINIZ; PAVANI, CAREN; BORZI, MARIANA MONEZI; OKINO, CINTIA HIROMI; MONTASSIER, HELIO JOSE. Memory immune responses and protection of chickens against a nephropathogenic infectious bronchitis virus strain by combining live heterologous and inactivated homologous vaccines. JOURNAL OF VETERINARY MEDICAL SCIENCE, v. 81, n. 4, p. 612-619, APR 2019. (01/14950-3)