Mechanisms of arterial pressure modulation through bradykinin in the bulbar medula - participation of paratrigeminal nucleus

• Grant number: 94/00693-3
• Support Opportunities: Research Projects - Thematic Grants
• Start date: January 01, 1996
• End date: April 30, 2000
• Field of knowledge: Biological Sciences - Pharmacology - Cardiorenal Pharmacology
• Principal Investigator: Charles Julian Lindsey
• Grantee: Charles Julian Lindsey
• Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil
• Associated research grant(s): 99/09150-6 - Electrophysiological evidence of bradikinin (bk) involving cardiovascular control in medula., AR.EXT; 96/04927-4 - 1) projections from the paratrigeminal nucleus to the medullary structures envolved in the arterial blood presure control in rats., AR.EXT

Abstract

The goal of the project is to investigate the bulbar mechanisms involved in the pressure response to bradykinin. The principal objectives are: a) to obtain a precise location of the bradykinin receptors with the technique of in situ hybridization; b) to obtain a mapping of the areas of projection of the paratrigeminal nucleus with the use of anterograde and retrograde markers; c) to obtain information on the functional relationship of paratrigeminal nucleus with the other structures involved in cardiovascular control by means of electrophysiological recordings and pharmacological blocking of the bulbar structures; d) to investigate the expression of the receptors in mice with genetically determined hypertension through the amplification and sequencing of the mRNA obtained from the tissue of different bulbar structures. (AU)