Crystallisation and protein crystallographic studies of a number of biologically important proteins.

• Grant number: 96/02285-5
• Support Opportunities: Regular Research Grants
• Start date: September 01, 1996
• End date: October 31, 1998
• Field of knowledge: Biological Sciences - Biochemistry - Molecular Biology
• Principal Investigator: Igor Polikarpov
• Grantee: Igor Polikarpov
• Host Institution: Associação Brasileira de Tecnologia de Luz Síncrotron (ABTLuS). Ministério da Ciência, Tecnologia e Inovação (Brasil). Campinas , SP, Brazil
• Associated scholarship(s): 97/13891-6 - Crystallisation and protein crystallographic studies of a number of biologically important proteins, BP.TT

Abstract

The processes of life depend fundamentally on the interactions and geometrical three-dimensional (3D) structure of the molecules in the living cell. To determine 3D structures the only general method available is X-ray diffraction. The project aims to determine the high resolution crystal structures of several biologically important enzymes such as lysine-ketoglutarate reductase-saccacharopine dehydrogenase, opaque2, glucosamine-6-phosphate synthase, beta-lactoglobulin, desulforedoxin and investigate their structure-function relationships. The proteins will be crystallized using random search method as well as standard techniques of variation of precipitant concentration over a wide range of pH's and their structures will be resolved with use of synchrotron radiation. (AU)